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Immunohistochemistry (Paraffin-embedded sections) - Anterior Gradient 2 antibody (ab43043)
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Western blot - Anterior Gradient 2 antibody (ab43043)
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Immunocytochemistry/ Immunofluorescence - Anterior Gradient 2 antibody (ab43043)
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Product Name
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Anterior Gradient 2 antibody
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See all Anterior Gradient 2 antibodies (7)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Anterior Gradient 2
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Immunogen
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Recombinant fusion protein (Human)
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Reacts with
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Hu
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Tested applications
(see key)
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ICC/IF, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 4 - 8 µg/ml.
IHC-P: Use at a concentration of 2 - 8 µg/ml.
WB: Use at a concentration of 2 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Secreted
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Research areas
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Developmental Biology >> Lineage specification >> Ectoderm
Cancer >> Tumor biomarkers >> Other
Stem Cells >> Lineage Markers >> Ectoderm
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Relevance
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Anterior gradient 2 (AGR2) and Anterior gradient 3 (AGR3) are recently discovered human homologues of the secreted Xenopus laevis proteins XAG-1/2 (AGR-1/2) that are expressed in the cement gland, an ectodermal organ in the head associated with anteroposterior fate determination during early development. Both AGR2 and AGR3 share a high degree of protein sequence homology and lie adjacent to one another on chromosome 7p21. AGR2 is expressed in a range of tumors including lung and breast tumors.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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IgG fraction
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Storage buffer
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Preservative: None
Constituents: Tris
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Form
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Liquid
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Concentration
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3.100 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Anterior Gradient 2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Anterior Gradient 2 antibody images, references, products related to ab43043 and other tools.
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Anterior Gradient 2 antibody images:
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 Immunohistochemistry (Paraffin-embedded sections) - Anterior Gradient 2 antibody (ab43043)
Immunohistochemistry of AGR2 in human tissues prepared from paraffin-embedded tissue blocks. Rabbit anti-AGR2 antibody ab43043 was used as primary antibody at a dilution range of 2 ug/ml to 4 ug/ml.
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Western blot - Anterior Gradient 2 antibody (ab43043)
All lanes : Anterior Gradient 2 antibody (ab43043) at 2 µg/ml
Lane 1 : HeLa cell extract
Lane 2 : PC3 cell extract
Lysates/proteins at 20 µg per lane.
Predicted band size : 20 kDa
The top panel is blotted with ab43043 anterior gradient 2 antibody and the lower panel with an anti-actin antibody as a loading control.
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Immunocytochemistry/ Immunofluorescence - Anterior Gradient 2 antibody (ab43043)
ab43043 staining cells from a human pancreatic cell line by ICC/IF. Cells were PFA fixed and permeabilized in 0.1% Triton X-100 prior to blocking in 1% BSA for 30 minutes at 22°C. The primary antibody was diluted 1/350 and incubated with the sample for 1 hour at 22°C. An Alexa Fluor® 488 conjugated donkey anti-rabbit antibody, diluted 1/1000, was used as the secondary. This image is courtesy of an anonymous Abreview
See Abreview
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References for Anterior Gradient 2 antibody (ab43043)
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This product has been used in:
Zhang JS et al. AGR2, an androgen-inducible secretory protein overexpressed in prostate cancer. Genes Chromosomes Cancer 43:249-59 (2005). PubMed: 15834940
If you publish research using ab43043 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Anterior Gradient 2
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