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Products:Stem Cells >> Lineage Markers >> Ectoderm
Overview
Product name
Anti-Anterior Gradient 2 antibody
See all Anterior Gradient 2 products (8) ...
Description
Rabbit polyclonal to Anterior Gradient 2
Tested applications
WB, ICC/IF, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fusion protein (Human)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: Tris
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Lineage specification >> Ectoderm
Cancer >> Tumor biomarkers >> Other
Stem Cells >> Lineage Markers >> Ectoderm
Applications
Show applications key
Our Abpromise guarantee covers the use of ab43043 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 2 µg/mlDetects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).
ICC/IF: Use a concentration of 4 - 8 µg/ml.
IHC-P: Use a concentration of 2 - 8 µg/ml.
Target
Function
Required for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells (By similarity). Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth.
Tissue specificity
Expressed strongly in trachea, lung, stomach, colon, prostate and small intestine. Expressed weakly in pituitary gland, salivary gland, mammary gland, bladder, appendix, ovary, fetal lung, uterus, pancreas, kidney, fetal kidney, testis, placenta, thyroid gland and in estrogen receptor (ER)-positive breast cancer cell lines.
Sequence similarities
Belongs to the AGR family.
Cellular localization
Secreted. Endoplasmic reticulum.
Target information above from: UniProt accessionO95994
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AG2 protein antibody
- hAG2 antibody
- Secreted cement gland protein XAG2 homolog antibody
see all
Database links
- Entrez Gene: 10551 Human
- Omim: 606358 Human
- SwissProt: O95994 Human
- Unigene: 530009 Human
Anti-Anterior Gradient 2 antibody images:
Immunohistochemistry (Paraffin-embedded sections) - Anterior Gradient 2 antibody (ab43043)
Immunohistochemistry of AGR2 in human tissues prepared from paraffin-embedded tissue blocks. Rabbit anti-AGR2 antibody ab43043 was used as primary antibody at a dilution range of 2 ug/ml to 4 ug/ml.
Western blot - Anterior Gradient 2 antibody (ab43043)
All lanes : Anti-Anterior Gradient 2 antibody (ab43043) at 2 µg/ml
Lane 1 : HeLa cell extract
Lane 2 : PC3 cell extract
Lysates/proteins at 20 µg per lane.
Predicted band size : 20 kDa
The top panel is blotted with ab43043 anterior gradient 2 antibody and the lower panel with an anti-actin antibody as a loading control.
Immunocytochemistry/ Immunofluorescence - Anterior Gradient 2 antibody (ab43043)
ab43043 staining cells from a human pancreatic cell line by ICC/IF. Cells were PFA fixed and permeabilized in 0.1% Triton X-100 prior to blocking in 1% BSA for 30 minutes at 22°C. The primary antibody was diluted 1/350 and incubated with the sample for 1 hour at 22°C. An Alexa Fluor® 488 conjugated donkey anti-rabbit antibody, diluted 1/1000, was used as the secondary.
This image is courtesy of an anonymous Abreview
References for Anti-Anterior Gradient 2 antibody (ab43043)
This product has been referenced in:
- Dumartin Let al. AGR2 is a novel surface antigen that promotes the dissemination of pancreatic cancer cells through regulation of cathepsins B and D. Cancer Res 71:7091-102 (2011). WB; Human.Read more (PubMed: 21948970) »
See 1 publication for this product
Publishing research using ab43043? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"