Overview

  • Product nameAntibody Purification Kit (Protein A)See all Purification kits ...
  • Product overview

    Commercially available antibodies often contain substances (e.g. BSA, glycine, tris, azide) that interfere in labeling reactions with enzymes or fluorophores. ab102784 quickly removes these contaminants. It can also be used to purify antibodies from crude samples such as ascites fluid or immune serum. The antibody to be purified or cleaned up  is ideally in a volume of 100-500µl. 

    Up to 500µg of antibody can be purified in each run.

    The method involves capture of the antibody on protein A resin and the removal of unwanted substances by a simple wash procedure, which is carried out in a standard microfuge. The purified product is then eluted and neutralized. Note: This method cannot be used with samples containing relatively dilute antibody in large volumes (e.g. tissue culture supernatant). For larger volumes, we recommend the use of Antibody TCS Purification Kit (3 purifications) (ab109207) or Antibody Serum Purification kit (3 purifications) (ab109209).

    The components of ab102784 are fully compatible with our Antibody conjugation kits.

     

    ab102784 is not suitable for goat antibody pufication.

Properties

  • Storage instructionsStore at +4°C. Please refer to protocols.
  • Components Identifier 3 tests 1 tests
    10x Binding Buffer 1 unit 1 unit
    Additional Collecting Tubes 4 units 1 unit
    Elution Buffer 1 unit 1 unit
    Neutralizer 1 vial 1 vial
    Protein A resin White cap 3 vials 1 vial
    Spin Cartridge/ Collecting tube assembly 1 unit 1 unit
    Wash Buffer 1 unit 1 unit
  • Research Areas

    Protocols

    References for Antibody Purification Kit (Protein A) (ab102784)

    This product has been referenced in:
    • Taoufiq Z  et al. Rho-kinase accelerates synaptic vesicle endocytosis by linking cyclic GMP-dependent protein kinase activity to phosphatidylinositol-4,5-bisphosphate synthesis. J Neurosci 33:12099-104 (2013). Read more (PubMed: 23864695) »

    See 1 Publication for this product

    Product Wall



    In cases where the antibody volume is larger, i.e. in your case where you have 1ml of antibody, we recommend you do the following:



    First, add the binding buffer to your antibody. Then add the binding buffer and antibody mix...

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    Protein values should be determined using an absorbance at 280nm. I have checked with our scientists, and they informed me that an absorbance (or OD) of 1 at 280nm corresponds to an antibody concentration of 0.1714mg/ml. Please note this value is ...

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    Thank you for contacting us.

    I am afraid that this kit doesn’t work for purification of IgMs. IgMs are normally purified by precipitation.

    I hope this information is helpful to you. Please do not hesitate to contact us if you ne...

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    As we discussed, our studies have found little effect on the conjugation chemistry with up to 0.05% BSA and up to 0.1% glycine in the antibody storage buffer. Thus, I think you do not have to worry about those components. However, as you know the ...

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    This kit will certainly suit your needs for removing the Tris-glycine and glycerol from your antibody before use with the EasyLink conjugation kit. While the EasyLink kits are able to be used with as much as 50% glycerol content they do need Tris level...

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    Thank you for your enquiry.

    We would recommend to aim for a maximum of 15,000g. Lower speeds will also be fine, it just may require slightly longer spin times to achieve the objective.

    I hope this will help. If you require any fur...

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    Thank you once again for your reply and patience.

    I am pleased to now have further information for you. We would recommend to use the following steps in this case:

    Add the resin to the 1 ml of antibody in a 1.5 ml eppendorf. Then ...

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    Thank you for contacting Abcam.

    Please find the link to the antibody purification kit below, that will allow you to remove the sodium azide from your antibody solution:

    http://www.abcam.com/antibody-purification-kit-protein-a-ab1027...

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    Thank you for bringing this to our attention. Assuming you followed the incubation and elution protocol, I am not sure what could have gone wrong. We have not had any complaints in the past several months. Did you try getting an absorbance reading for ...

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    Thank you for contacting us.

    I have checked with the lab and they sent me the following explanation:

    TheElution Buffer is used to elute (release) the purified IgG from the Protein A resin. The Elution Buffer works by low pH, and is...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"