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Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

Submit an Abreview Q&A (2)Specific References (1)

Overview

  • Product nameAntigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) See all Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) reagents ...
  • Description
    Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0)
  • Tested applicationsIHC-P more details

    • Properties

  • FormLiquid
  • Storage instructionsStore at room temperature.
  • Storage bufferpH: 9.00
    Constituents: 5% Tris, 1.5% 2-butoxyethanol, 5% Sodium EDTA
    Note: Buffer 100x concentrated with detergent.
  • Concentration information loading...
  • Research Areas
  • RelevanceAntigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) is a special solution that enables rehydrate and target retrieval in formalin-fixed, paraffin-embedded tissue sections in one step. It is optimal for use with primary antibodies that require Tris-EDTA buffer (pH=9.0) pretreatment.

    • Applications

    Our Abpromise guarantee covers the use of ab93684 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    IHC-P IHC-P: Use at an assay dependent dilution.

    References for Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

    This product has been referenced in:
    • Li Y  et al. Perinatal nicotine exposure increases vulnerability of hypoxic-ischemic brain injury in neonatal rats: role of angiotensin II receptors. Stroke 43:2483-90 (2012). Read more (PubMed: 22738920) »

    See 1 Publication for this product

    Publishing research using ab93684 ? Please let us know so that we can cite the reference in this datasheet

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    Submit an Abreview for ab93684 Submit a Question for ab93684

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     I would like to inform you that I plan to use Peroxide/DAB the substrate and chromogen for horseradish peroxidase as detection system I appreciate you for your kind help                                                       with my best regards      ...

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    Thank you for your reply. I recommend the following HRP conjugated secondary antibodies to use with these rabbit Anti-GAD65+GAD67 primary antibodies. I have included one product from a donkey host and another from goat. Both are guaranteed and the choi...

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    What protocol do you use with this product?

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    Please find the recommended protocol to use with ab93684 attached.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"