Antithrombin III 293T Transfected Lysate - (positive control) (ab94043)

Overview

  • Product nameAntithrombin III 293T Transfected Lysate - (positive control)
  • General notesab94043 is a 293T cell transfected lysate in which Human Antithrombin III has been transiently over-expressed using a pCMV-Antithrombin III plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details on how the transfected lysate was prepared view preparation notes
  • Tested applicationsWBmore details

Properties

  • BackgroundDisease: Defects in SERPINC1 are the cause of antithrombin III deficiency (AT3D) [MIM:613118]. AT3D is an important risk factor for hereditary thrombophilia, a hemostatic disorder characterized by a tendency to recurrent thrombosis. AT3D is classified into 4 types. Type I: characterized by a 50% decrease in antigenic and functional levels. Type II: has defects affecting the thrombin-binding domain. Type III: alteration of the heparin-binding domain. Plasma AT-III antigen levels are normal in type II and III. Type IV: consists of miscellaneous group of unclassifiable mutations. Function: Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin. PTM: Phosphorylation sites are present in the extracelllular medium. Similarity: Belongs to the serpin family. Tissue specificity: Found in plasma.

Applications

Our Abpromise guarantee covers the use of ab94043 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Antithrombin III 293T Transfected Lysate - (positive control) images

    • ab94043 at 15µg/lane on an SDS-PAGE gel.
    • All lanes : Anti-Antithrombin III antibody (ab66234) at 1/500 dilution

      Lane 1 : Antithrombin III 293T Transfected Lysate - (positive control) (ab94043)
      Lane 2 : 293T non-transfected lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

    References for Antithrombin III 293T Transfected Lysate - (positive control) (ab94043)

    ab94043 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"