Antithrombin III Human ELISA Kit (ab108801)
Overview
- Product nameAntithrombin III Human ELISA KitSee all Antithrombin III kits ...
- Detection methodColorimetric
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Saliva, Milk, Urine
- Assay typeSandwich
- Sensitivity= 3 ng/ml
- Range3.13 ng/ml - 200 ng/ml
- Recovery
99.5 %
- Assay time4h 0m
- Assay durationMultiple steps standard assay
- Species reactivityReacts with: Human
- Product overview
The serine-protease-inhibitor antithrombin III (AT III), the most important natural inhibitor of thrombin activity, has been shown to exert marked anti-inflammatory properties and proven to be efficacious in experimental models of sepsis, septic shock, and disseminated intravascular coagulation. It has often been recommended for the therapy of septic patients as it provides anticoagulant and anti-inflammatory actions. AT III deficiency is a rare hereditary disease that predisposes to throm-boembolic complications. AT III levels are positively correlated with plasma total cholesterol levels, plasma low-density lipoprotein cholesterol levels, plasma triglycerides and D-dimer levels.
ab108801 Antithrombin III Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of human AT III in urine, milk, saliva, and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique, which measures AT III in less than 4 hours. A monoclonal antibody specific for AT III has been pre-coated onto a microplate. AT III in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for AT III, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
- Tested applicationsELISA more details
- PlatformMicroplate
Properties
- Storage instructionsStore at +4°C. Please refer to protocols.
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Components 1 x 96 tests Biotinylated Human Antithrombin III Antibody (50x) 1 x 140µl Chromogen Substrate 1 x 8ml Diluent Concentrate (10x) 1 x 30ml Human Antithrombin III Microplate 1 x 96 wells 1 unit Human Antithrombin III Standard 1 unit Stop Solution 1 x 12ml Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 80µl Wash Buffer Concentrate (20x) 2 x 30ml -
Research Areas
- SwissProt: P01008 Human
Applications
Our Abpromise guarantee covers the use of ab108801 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ELISA | ELISA |
Antithrombin III Human ELISA Kit images
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ELISA - Antithrombin III Human ELISA Kit - 1 x 96 Well Plate (ab108801)Antithrombin III standard curve.
Protocols
References for Antithrombin III Human ELISA Kit (ab108801)
ab108801 has not yet been referenced specifically in any publications.
