Antithrombin III Human ELISA Kit (ab108801)
- Product nameAntithrombin III Human ELISA KitSee all Antithrombin III kits ...
- Detection methodColorimetric
Intra-assay Sample n Mean SD CV% Overall 4.6%
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Saliva, Milk, Urine
- Assay typeSandwich (quantitative)
- Sensitivity= 3 ng/ml
- Range3.13 ng/ml - 200 ng/ml
- Assay time4h 0m
- Assay durationMultiple steps standard assay
- Species reactivityReacts with: Human
- Product overview
Abcam’s Antithrombin III Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Antithrombin III in urine, milk, saliva, and cell culture supernatants.
An antithrombin III specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an antithrombin III specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of antithrombin III captured in plate.
- Tested applicationsSandwich ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 70X Biotinylated Human Antithrombin III Antibody 1 x 105µl Antithrombin III Microplate (12 x 8 well strips) 1 unit Antithrombin III Standard 1 vial Chromogen Substrate 1 x 8ml Sealing Tapes 3 units Stop Solution 1 x 12ml
- SwissProt: P01008 Human
Our Abpromise guarantee covers the use of ab108801 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Antithrombin III Human ELISA Kit (ab108801)
ab108801 has not yet been referenced specifically in any publications.