Anti-Antithrombin III antibody [8D5] (ab117851)

Overview

  • Product nameAnti-Antithrombin III antibody [8D5]See all Antithrombin III primary antibodies ...
  • Description
    Mouse monoclonal [8D5] to Antithrombin III
  • Tested applicationsFlow Cyt, WB more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length recombinant Human Antithrombin III (NP_000479) produced in HEK293T cells.

  • Positive control
    • HEK293T transfected cells

Properties

  • FormLiquid
  • Storage instructionsStore at -20°C. Stable for 12 months at -20°C
  • Storage bufferpH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 1% BSA, 50% Glycerol, 48% PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified from mouse ascite fluid by affinity chromatography
  • Clonality Monoclonal
  • Clone number8D5
  • IsotypeIgG2b
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab117851 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Flow Cyt 1/100.
WB Use at an assay dependent concentration. Predicted molecular weight: 52 kDa.

Target

  • FunctionMost important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin.
  • Tissue specificityFound in plasma.
  • Involvement in diseaseDefects in SERPINC1 are the cause of antithrombin III deficiency (AT3D) [MIM:613118]. AT3D is an important risk factor for hereditary thrombophilia, a hemostatic disorder characterized by a tendency to recurrent thrombosis. AT3D is classified into 4 types. Type I: characterized by a 50% decrease in antigenic and functional levels. Type II: has defects affecting the thrombin-binding domain. Type III: alteration of the heparin-binding domain. Plasma AT-III antigen levels are normal in type II and III. Type IV: consists of miscellaneous group of unclassifiable mutations.
  • Sequence similaritiesBelongs to the serpin family.
  • Post-translational
    modifications
    Phosphorylation sites are present in the extracelllular medium.
  • Cellular localizationSecreted > extracellular space.
  • Target information above from: UniProt accession P01008 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • ANT3_HUMAN antibody
    • Antithrombin antibody
    • Antithrombin III antibody
    • Antithrombin-III antibody
    • AntithrombinIII antibody
    • AT 3 antibody
    • AT III antibody
    • AT3 antibody
    • AT3D antibody
    • ATIII antibody
    • Heparin cofactor I antibody
    • MGC22579 antibody
    • Serine (or cysteine) proteinase inhibitor clade C (antithrombin) member 1 antibody
    • Serine cysteine proteinase inhibitor clade C member 1 antibody
    • Serine proteinase inhibitor clade C member 1 antibody
    • Serpin C1 antibody
    • Serpin peptidase inhibitor clade C (antithrombin) member 1 antibody
    • SERPINC1 antibody
    • THPH7 antibody
    see all

Anti-Antithrombin III antibody [8D5] images

  • Flow cytometry image using ab117851 at a 1/100 dilution, staining HEK293T cells transfected with Antithrombin III (Red), or an empty vector control plasmid (Blue).

References for Anti-Antithrombin III antibody [8D5] (ab117851)

ab117851 has not yet been referenced specifically in any publications.

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Ab19170, ab46533 and ab117851 have all been validated in Flow Cyt and therefore are guaranteed to work in this application.

Regarding ab19170, cells were were fixed with 4% paraformaldehyde but not perm...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"