Anti-Antizyme inhibitor 1 antibody [76.1_1E5] (ab127974)
Overview
- Product nameAnti-Antizyme inhibitor 1 antibody [76.1_1E5]See all Antizyme inhibitor 1 primary antibodies ...
- DescriptionMouse monoclonal [76.1_1E5] to Antizyme inhibitor 1
- Specificityab127974 specificity has been determined by protein microarray.
- Tested applicationsWB, ICC/IF more details
- Species reactivityReacts with: Human
- Immunogen
HeLa cells.
- Positive controlTransfected HeLa cells.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Store at -20ºC.
- Storage bufferPreservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading... - PurityIgG fraction
- Clonality Monoclonal
- Clone number76.1_1E5
- IsotypeIgG2b
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab127974 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/1000. Predicted molecular weight: 50 kDa. |
| ICC/IF | ICC/IF: 1/100. 4% paraformaldehyde fixed, 0.1% Triton X-100 permeabilized |
Target
- FunctionRegulates cellular polyamine homeostasis (By similarity). Inhibits antizyme-dependent ornithine decarboxylase degradation by binding to antizyme.
- Tissue specificityExpressed in liver.
- Sequence similaritiesBelongs to the Orn/Lys/Arg decarboxylase class-II family. ODC antizyme inhibitor subfamily.
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Database links
- Entrez Gene: 51582 Human
- Omim: 607909 Human
- SwissProt: O14977 Human
- Unigene: 459106 Human
Target information above from: UniProt accession
O14977
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- Antizyme inhibitor 1 antibodyAZI antibodyAzin1 antibody
- AZIN1_HUMAN antibodyOAZI antibodyOAZIN antibodyODC1L antibodyOrnithine decarboxylase antizyme inhibitor antibody
see all
Anti-Antizyme inhibitor 1 antibody [76.1_1E5] images
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![Immunocytochemistry/ Immunofluorescence - Anti-Antizyme inhibitor 1 antibody [76.1_1E5] (ab127974)](http://a.abcam.com/ps/datasheet/images/127/ab127974/Antizyme-inhibitor-1-Primary-antibodies-ab127974-1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Antizyme inhibitor 1 antibody [76.1_1E5] (ab127974)ab127974 at 1/100 staining Antizyme inhibitor 1 in 4% paraformaldehyde fixed, 0.1% Triton X-100 permeabilized HeLa cells by Immunocytochemistry. -
![Western blot - Anti-Antizyme inhibitor 1 antibody [76.1_1E5] (ab127974)](http://a.abcam.com/ps/datasheet/images/127/ab127974/Antizyme-inhibitor-1-Primary-antibodies-ab127974-2.jpg)
Western blot - Anti-Antizyme inhibitor 1 antibody [76.1_1E5] (ab127974)Lanes 1 - 3 : Anti-Antizyme inhibitor 1 antibody [76.1_1E5] (ab127974) at 1/1000 dilution
Lanes 4 - 6 : V5 antibody control at 1/1000 dilution
Lane 1 : Transfected HeLa cell lysate
Lane 2 : Non-Transfected HeLa cell lysate
Lane 3 : SH-SY5Y cell lysate
Lane 4 : Transfected HeLa cell lysate
Lane 5 : Non-Transfected HeLa cell lysate
Lane 6 : SH-SY5Y cell lysate
Predicted band size : 50 kDa
References for Anti-Antizyme inhibitor 1 antibody [76.1_1E5] (ab127974)
ab127974 has not yet been referenced specifically in any publications.
