Overview
- Product nameAnti-Apc10 antibodySee all Apc10 primary antibodies ...
- DescriptionRabbit polyclonal to Apc10
- Specificityab106497 is predicted not to cross-react with other APC family members. Two isoforms of Apc10 are known to exist; ab106497 will recognize both isoforms.
- Tested applicationsICC/IF, WB, IHC-P more details
- Species reactivityReacts with: Mouse, Rat, Human
- Immunogen
A 16 amino acid synthetic peptide near the center of Human Apc10 (EAX05052).
- Positive controlMouse heart tissue and lysate
Properties
- FormLiquid
- Storage instructionsStore at 4°C, stable for one year. Should not be exposed to prolonged high temperatures. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: PBS -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab106497 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ICC/IF | ICC/IF: Use a concentration of 20 µg/ml. |
| WB | WB: Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 21 kDa. |
| IHC-P | IHC-P: Use at an assay dependent dilution. |
Target
- FunctionComponent of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
- PathwayProtein modification; protein ubiquitination.
- Sequence similaritiesBelongs to the APC10 family.
Contains 1 DOC domain. -
Database links
- Entrez Gene: 10393 Human
- Entrez Gene: 68999 Mouse
- Entrez Gene: 361389 Rat
- Omim: 613745 Human
- SwissProt: Q9UM13 Human
- SwissProt: Q8K2H6 Mouse
- Unigene: 480876 Human
- Unigene: 257445 Mouse
- Unigene: 420790 Mouse
- Unigene: 9619 Rat
see all
Target information above from: UniProt accession
Q9UM13
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- ANAPC 10 antibodyanapc10 antibodyAnaphase promoting complex 10 antibody
- Anaphase promoting complex subunit 10 antibodyAnaphase-promoting complex subunit 10 antibodyApc 10 antibodyAPC10 antibodyAPC10_HUMAN antibodyCyclosome subunit 10 antibodyDKFZP564L0562 antibodyDoc 1 antibodyDoc1 antibodyDoc1, S. cerevisiae, homolog of antibodyOTTHUMP00000220297 antibodyOTTHUMP00000220298 antibodyOTTHUMP00000220299 antibodyOTTHUMP00000220300 antibodyOTTHUMP00000220303 antibody
see all
Anti-Apc10 antibody images
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Western blot - Apc10 antibody (ab106497)Lane 1 : Anti-Apc10 antibody (ab106497) at 1 µg/ml
Lane 2 : Anti-Apc10 antibody (ab106497) at 2 µg/ml
Lane 1 : Mouse heart tissue lysate
Lane 2 : Mouse heart tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size : 21 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Apc10 antibody (ab106497)ab106497, at 5 µg/ml, staining Apc10 in Mouse heart tissue by Immunohistochemistry.
References for Anti-Apc10 antibody (ab106497)
ab106497 has not yet been referenced specifically in any publications.
