Overview

  • Product nameAnti-Apc10 antibody
    See all Apc10 primary antibodies
  • Description
    Rabbit polyclonal to Apc10
  • Specificityab106497 is predicted not to cross-react with other APC family members. Two isoforms of Apc10 are known to exist; ab106497 will recognize both isoforms.
  • Tested applicationsICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A 16 amino acid synthetic peptide near the center of Human Apc10 (EAX05052).

  • Positive control
    • Mouse heart tissue and lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at 4°C (stable for up to 12 months).
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab106497 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 21 kDa.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionComponent of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the APC10 family.
    Contains 1 DOC domain.
  • Information by UniProt
  • Database links
  • FormIt localises to Cytosol and nucleoplasm.
  • Alternative names
    • ANAPC 10 antibody
    • anapc10 antibody
    • Anaphase promoting complex 10 antibody
    • Anaphase promoting complex subunit 10 antibody
    • Anaphase-promoting complex subunit 10 antibody
    • Apc 10 antibody
    • APC10 antibody
    • APC10_HUMAN antibody
    • Cyclosome subunit 10 antibody
    • DKFZP564L0562 antibody
    • Doc 1 antibody
    • Doc1 antibody
    • Doc1, S. cerevisiae, homolog of antibody
    • OTTHUMP00000220297 antibody
    • OTTHUMP00000220298 antibody
    • OTTHUMP00000220299 antibody
    • OTTHUMP00000220300 antibody
    • OTTHUMP00000220303 antibody
    see all

Anti-Apc10 antibody images

  • Immunofluorescence of Apc10 in mouse heart tissue with ab106497 at 20 ug/mL.

  • Lane 1 : Anti-Apc10 antibody (ab106497) at 1 µg/ml
    Lane 2 : Anti-Apc10 antibody (ab106497) at 2 µg/ml

    Lane 1 : Mouse heart tissue lysate
    Lane 2 : Mouse heart tissue lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 21 kDa
  • ab106497, at 5 µg/ml, staining Apc10 in Mouse heart tissue by Immunohistochemistry.

References for Anti-Apc10 antibody (ab106497)

ab106497 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (HCT116 cell lines)
Gel Running Conditions Reduced Denaturing (4-12%)
Loading amount 30 µg
Specification HCT116 cell lines
Blocking step odyssey PBS as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: 37°C
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Submitted Jul 14 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"