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Apc2 293T Transfected Lysate - (positive control) (ab94319)

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Overview

  • Product nameApc2 293T Transfected Lysate - (positive control)
  • General notesab94319 is a 293T cell transfected lysate in which Human Apc2 has been transiently over-expressed using a pCMV-Apc2 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsWB more details

Properties

  • BackgroundFunction: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Pathway: Protein modification; protein ubiquitination. Similarity: Belongs to the cullin family.

    • Applications

    Our Abpromise guarantee covers the use of ab94319 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB
  • Application notesWB: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Apc2 293T Transfected Lysate - (positive control) images

    • SDS-PAGE - Apc2 293T Transfected Lysate - (positive control) (ab94319)
      SDS-PAGE - Apc2 293T Transfected Lysate - (positive control) (ab94319)
      ab94319 at 15µg/lane on an SDS-PAGE gel.
    • Western blot - Apc2 293T Transfected Lysate - (positive control) (ab94319)
      Western blot - Apc2 293T Transfected Lysate - (positive control) (ab94319)
      All lanes : Anti-Apc2 antibody (ab57094) at 1/500 dilution

      Lane 1 : Apc2 293T Transfected Lysate - (positive control) (ab94319)
      Lane 2 : 293T non-transfected lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

    References for Apc2 293T Transfected Lysate - (positive control) (ab94319)

    ab94319 has not yet been referenced specifically in any publications.

    Publishing research using ab94319 ? Please let us know so that we can cite the reference in this datasheet

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"