Anti-Apc4 antibody (ab113364)
Key features and details
- Rabbit polyclonal to Apc4
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Apc4 antibody
See all Apc4 primary antibodies -
Description
Rabbit polyclonal to Apc4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide conjugated to KLH, corresponding to N terminal region of Human Apc4.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab113364 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 92 kDa.
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 92 kDa. |
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the APC4 family. - Information by UniProt
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Database links
- Entrez Gene: 29945 Human
- Entrez Gene: 52206 Mouse
- Entrez Gene: 305420 Rat
- Omim: 606947 Human
- SwissProt: Q9UJX5 Human
- SwissProt: Q91W96 Mouse
- Unigene: 152173 Human
- Unigene: 272568 Mouse
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Alternative names
- ANAPC 4 antibody
- anapc4 antibody
- Anaphase Promoting Complex Subunit 4 antibody
see all
Images
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ab113364, at 5µg/ml, staining Apc4 in Formalin-fixed, Paraffin-embedded Human Spleen tissue by Immunohistochemistry.
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ab113364, at 5µg/ml, staining Apc4 in Formalin-fixed, Paraffin-embedded Human uterus tissue by Immunohistochemistry.
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Lane 1 : Anti-Apc4 antibody (ab113364) at 1 µg/ml
Lane 2 : Anti-Apc4 antibody (ab113364) at 2 µg/ml
All lanes : Mouse liver tissue lysate
Predicted band size: 92 kDa
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab113364 has not yet been referenced specifically in any publications.