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Immunofluorescence - Apc5 antibody (ab4170)
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Product Name
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Apc5 antibody
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See all Apc5 antibodies (5)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Apc5
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Immunogen
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Synthetic peptide derived from residues 250 - 350 of Human Apc5 conjugated to KLH.
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Reacts with
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Hu
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Tested applications
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ICC/IF
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Abreviews
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Application notes
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Recommended dilutions
ICC/IF: 1/100.
To date we have been unable to detect a band of the correct size (~80kDa) by Western blotting using HeLa nuclear and HeLa whole cell lysates. We are uncertain if this is because the antibody is not suitable for use in Western blot, or because the lysates we are using are not the most appropriate.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Cell Biology >> Cell Cycle >> Cell Division >> Other cell division antibodies
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Other E3 Ligases
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> APC/C
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Relevance
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Apc5 is a subunit of the APC / Cyclosome. APC5 (anaphase-promoting complex subunit 5) is a member of the E3 enzyme family. The APC5 protein is located in the nucleus during interphase and at the centrosome during metaphase/anaphase. This protein functions with other members of the APC complex as a multisubunit cell cycle ubiquitin ligase, and a regulator of sister chromatid separation by degrading securins. In addition, this protein functions in ubiquitin-dependent cyclin catabolism, metaphase/anaphase transition, and spindle elongation. The APC5 protein comprises one subunit of the anaphase promoting complex including APC1-8, and other probable complex proteins APC9-11, Cdc26, Mnd2, Swm1. The APC complex is inactivated by protein kinase A and is activated by CDC20 and Cdh1.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.400 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Apc5 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Apc5 antibody images, references, products related to ab4170 and other tools.
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Apc5 antibody images:
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 Immunofluorescence - Apc5 antibody (ab4170)
Metaphase HeLa cells labelled with APC5 (ab4170) and counterstained with DAPI.
The antibody shows foci of APC5 localised to the centrosomes. 100x magnification. Panel 1: DNA stained with DAPI
Panel 2: Cells stained with ab4170 (1/100 dilution)
Panel 3: DAPI staining is shown in red, ab4170 staining is shown in green Kirk McManus, University of British Columbia
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References for Apc5 antibody (ab4170)
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This product has been used in:
Tegha-Dunghu J et al. EML3 is a nuclear microtubule-binding protein required for the correct alignment of chromosomes in metaphase. J Cell Sci 121:1718-26 (2008). ICC/IF; Human. PubMed: 18445686
If you publish research using ab4170 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Apc5
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Apc5 antibody - more information
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Customer reviews (feedback) regarding Apc5 antibody |
Customer FAQs regarding Apc5 antibody |
Protocols for Apc5 antibody |
Price and availability of products related to Apc5 antibody |
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Apc5 antibody - related products:
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Apc5 antibody - other tools:
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Contact Abcam with a Technical Enquiry about Apc5 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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