Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> APC/C
Overview
Product name
Anti-Apc5 antibody
See all Apc5 products (6) ...
Description
Rabbit polyclonal to Apc5
Tested applications
IF, ICCmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide derived from residues 250 - 350 of Human Apc5 conjugated to KLH.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
-
Immunofluorescence - Apc5 antibody (ab4170)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab4170 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
IF
Application notes
ICC/IF: 1/100.
To date we have been unable to detect a band of the correct size (~80kDa) by Western blotting using HeLa nuclear and HeLa whole cell lysates. We are uncertain if this is because the antibody is not suitable for use in Western blot, or because the lysates we are using are not the most appropriate.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
Pathway
Protein modification; protein ubiquitination.
Sequence similarities
Belongs to the APC5 family.
Contains 4 TPR repeats.
Target information above from: UniProt accessionQ9UJX4
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AA408751 antibody
- AA536819 antibody
- AA986414 antibody
see all
Database links
- Entrez Gene: 51433 Human
- Omim: 606948 Human
- SwissProt: Q9UJX4 Human
- Unigene: 7101 Human
Anti-Apc5 antibody images:
Immunofluorescence - Apc5 antibody (ab4170)
Metaphase HeLa cells labelled with APC5 (ab4170) and counterstained with DAPI.
The antibody shows foci of APC5 localised to the centrosomes. 100x magnification.
Panel 1: DNA stained with DAPI
Panel 2: Cells stained with ab4170 (1/100 dilution)
Panel 3: DAPI staining is shown in red, ab4170 staining is shown in green
Kirk McManus, University of British Columbia
References for Anti-Apc5 antibody (ab4170)
This product has been referenced in:
- Tegha-Dunghu Jet al. EML3 is a nuclear microtubule-binding protein required for the correct alignment of chromosomes in metaphase. J Cell Sci 121:1718-26 (2008). ICC/IF; Human.Read more (PubMed: 18445686) »
See 1 publication for this product
Publishing research using ab4170? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"