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All lanes : Anti-Apc5 antibody (ab72516) at 0.4 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg
developed using the ECL technique
Predicted band size : 85 kDa
Observed band size : 85 kDa
Exposure time : 3 minutes
Immunoprecipitation/ Western Blot of Apc5. Lane 1: ab72516 at 3µg/mg Hela whole cell lysate (1mg for IP, 20% of IP loaded). Lane 2: Control IgG. ab72516 at 1µg/ml for WB. Chemiluminescence with an exposure time of 1 minute.
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