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Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> APC/C
Overview
Product name
Anti-Apc5 antibody
See all Apc5 products (6) ...
Description
Rabbit polyclonal to Apc5
Tested applications
WB, IPmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide from a region between residue 705 and the C terminus (residue 755) of human Apc5 (NP_057321.2)
Positive control
Whole cell lysate from Hela, 293T and NIH3T3 cells.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab72516 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/2500.Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).
IP: Use at 2-5 µg/mg of lysate.
Target
Function
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
Pathway
Protein modification; protein ubiquitination.
Sequence similarities
Belongs to the APC5 family.
Contains 4 TPR repeats.
Target information above from: UniProt accessionQ9UJX4
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AA408751 antibody
- AA536819 antibody
- AA986414 antibody
see all
Database links
- Entrez Gene: 51433 Human
- Entrez Gene: 59008 Mouse
- Omim: 606948 Human
- SwissProt: Q9UJX4 Human
- SwissProt: Q8BTZ4 Mouse
- Unigene: 7101 Human
- Unigene: 332667 Mouse
- Unigene: 359360 Mouse
Anti-Apc5 antibody images:
Western blot - Apc5 antibody (ab72516)
All lanes : Anti-Apc5 antibody (ab72516) at 0.4 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg
developed using the ECL technique
Predicted band size : 85 kDa
Observed band size : 85 kDa
Exposure time : 3 minutes
Immunoprecipitation - Apc5 antibody (ab72516)
Immunoprecipitation/ Western Blot of Apc5. Lane 1: ab72516 at 3µg/mg Hela whole cell lysate (1mg for IP, 20% of IP loaded). Lane 2: Control IgG. ab72516 at 1µg/ml for WB. Chemiluminescence with an exposure time of 1 minute.
References for Anti-Apc5 antibody (ab72516)
This product has been referenced in:
- Dai Let al. Dose-dependent proteomic analysis of glioblastoma cancer stem cells upon treatment with ?-secretase inhibitor. Proteomics : (2011). WB; Human.Read more (PubMed: 21932445) »
See 1 publication for this product
Publishing research using ab72516? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"