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  • Product nameAnti-Apc7 antibody
    See all Apc7 primary antibodies
  • Description
    Rabbit polyclonal to Apc7
  • Tested applicationsWB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human Apc7.

    (Peptide available as ab13734.)

  • Positive control
    • This antibody gave a positive control in the following lysates: HeLa Nuclear Extract Heart (Mouse) Tissue Spinal Cord (Mouse) Tissue



Our Abpromise guarantee covers the use of ab4171 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).Can be blocked with Apc7 peptide (ab13734).
ICC/IF Use a concentration of 10 µg/ml.


  • FunctionComponent of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the APC7 family.
    Contains 7 TPR repeats.
  • Information by UniProt
  • Database links
  • Alternative names
    • anapc7 antibody
    • Anaphase promoting complex subunit 7 antibody
    • Anaphase-promoting complex subunit 7 antibody
    • APC 7 antibody
    • APC7 antibody
    • APC7_HUMAN antibody
    • Cyclosome subunit 7 antibody
    • Prediabetic NOD sera reactive autoantigen antibody
    see all

Anti-Apc7 antibody images

  • All lanes : Anti-Apc7 antibody (ab4171)

    Lane 1 : HeLa Nuclear Extract
    Lane 2 : HeLa Nuclear Extract with Apc7 peptide (ab13734) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution
    Lane 2 : Goat polyclonal to Rabbit IgG - H&L (HRP) (ab6721) at 1/2000 dilution

    Predicted band size : 63 kDa
    Observed band size : 63 kDa
    Additional bands at : 39 kDa,45 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute
  • All lanes : Anti-Apc7 antibody (ab4171) at 1 µg/ml

    Lane 1 : Heart (Mouse) Tissue Lysate
    Lane 2 : Spinal Cord (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 63 kDa
    Observed band size : 63 kDa
    Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab4171 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4171, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Apc7 antibody (ab4171)

This product has been referenced in:
  • Izawa D & Pines J How APC/C-Cdc20 changes its substrate specificity in mitosis. Nat Cell Biol 13:223-33 (2011). WB ; Human . Read more (PubMed: 21336306) »
  • Nilsson J  et al. The APC/C maintains the spindle assembly checkpoint by targeting Cdc20 for destruction. Nat Cell Biol 10:1411-20 (2008). Read more (PubMed: 18997788) »

See all 2 Publications for this product

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