Overview

  • Product nameAnti-Apc7 antibodySee all Apc7 primary antibodies ...
  • Description
    Rabbit polyclonal to Apc7
  • Tested applicationsWB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human Apc7.

    (Peptide available as ab13734.)

  • Positive control
    • This antibody gave a positive control in the following lysates: HeLa Nuclear Extract Heart (Mouse) Tissue Spinal Cord (Mouse) Tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab4171 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).Can be blocked with Apc7 peptide (ab13734).
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionComponent of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the APC7 family.
    Contains 7 TPR repeats.
  • Information by UniProt
  • Database links
  • Alternative names
    • ANAPC7 antibody
    • Anaphase promoting complex subunit 7 antibody
    • Anaphase-promoting complex subunit 7 antibody
    • APC 7 antibody
    • APC7 antibody
    • APC7_HUMAN antibody
    • Cyclosome subunit 7 antibody
    • Prediabetic NOD sera reactive autoantigen antibody
    • Prediabetic NOD sera-reactive autoantigen antibody
    see all

Anti-Apc7 antibody images

  • All lanes : Anti-Apc7 antibody (ab4171)

    Lane 1 : HeLa Nuclear Extract
    Lane 2 : HeLa Nuclear Extract with Apc7 peptide (ab13734) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution
    Lane 2 : Goat polyclonal to Rabbit IgG - H&L (HRP) (ab6721) at 1/2000 dilution


    Predicted band size : 63 kDa
    Observed band size : 63 kDa
    Additional bands at : 39 kDa,45 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute
  • All lanes : Anti-Apc7 antibody (ab4171) at 1 µg/ml

    Lane 1 : Heart (Mouse) Tissue Lysate
    Lane 2 : Spinal Cord (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 63 kDa
    Observed band size : 63 kDa
    Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab4171 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4171, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Apc7 antibody (ab4171)

This product has been referenced in:
  • Izawa D & Pines J How APC/C-Cdc20 changes its substrate specificity in mitosis. Nat Cell Biol 13:223-33 (2011). WB ; Human . Read more (PubMed: 21336306) »
  • Nilsson J  et al. The APC/C maintains the spindle assembly checkpoint by targeting Cdc20 for destruction. Nat Cell Biol 10:1411-20 (2008). Read more (PubMed: 18997788) »

See all 2 Publications for this product

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