Anti-Apc7 antibody (ab4171)

Overview

• Product nameAnti-Apc7 antibodySee all Apc7 primary antibodies ...
• Description
  Rabbit polyclonal to Apc7
• Tested applicationsWB, ICC/IF more details
• Species reactivity
  Reacts with: Mouse, Human
  
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human Apc7.

  Read Abcam's proprietary immunogen policy

  (Peptide available as ab13734.)

• Positive controlThis antibody gave a positive control in the following lysates: HeLa Nuclear Extract Heart (Mouse) Tissue Spinal Cord (Mouse) Tissue

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • Cell cycle
  • Chromosome Structure
  • Chromatid Cohesion

  • Cell Biology
  • Proteolysis / Ubiquitin
  • Proteasome / Ubiquitin
  • APC/C

Applications

Target

• FunctionComponent of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
• PathwayProtein modification; protein ubiquitination.
• Sequence similaritiesBelongs to the APC7 family.
  Contains 7 TPR repeats.

Target information above from: UniProt accession Q9UJX3 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 51434 Human
  • Entrez Gene: 56317 Mouse
  • Omim: 606949 Human
  • SwissProt: Q9UJX3 Human
  • SwissProt: Q9WVM3 Mouse
  • Unigene: 719935 Human
  • Unigene: 37341 Mouse

• Alternative names
  ANAPC7 antibodyAnaphase promoting complex subunit 7 antibodyAnaphase-promoting complex subunit 7 antibody

  APC 7 antibodyAPC7 antibodyAPC7_HUMAN antibodyCyclosome subunit 7 antibodyPrediabetic NOD sera reactive autoantigen antibodyPrediabetic NOD sera-reactive autoantigen antibody

  see all

Anti-Apc7 antibody images

• 

  Western blot - Apc7 antibody (ab4171)
  All lanes : Anti-Apc7 antibody (ab4171)
  
  Lane 1 : HeLa Nuclear Extract
  Lane 2 : HeLa Nuclear Extract with Apc7 peptide (ab13734) at 1 µg/ml
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  Lane 1 : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721) at 1/2000 dilution
  Lane 2 : Goat polyclonal to Rabbit IgG - H&L (HRP) (ab6721) at 1/2000 dilution
  
  
  Predicted band size : 63 kDa
  Observed band size : 63 kDa
  Additional bands at : 39 kDa,45 kDa. We are unsure as to the identity of these extra bands.
  
  Exposure time : 1 minute
• 

  Western blot - Apc7 antibody (ab4171)
  All lanes : Anti-Apc7 antibody (ab4171) at 1 µg/ml
  
  Lane 1 : Heart (Mouse) Tissue Lysate
  Lane 2 : Spinal Cord (Mouse) Tissue Lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
  
  Performed under reducing conditions.
  
  Predicted band size : 63 kDa
  Observed band size : 63 kDa
  Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.
• 

  Immunocytochemistry/ Immunofluorescence - Anti-Apc7 antibody (ab4171)
  ICC/IF image of ab4171 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4171, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.