Overview
- Product nameAnti-Apg12 antibody [EPR4799]See all Apg12 primary antibodies ...
- DescriptionRabbit monoclonal [EPR4799] to Apg12
- Tested applicationsICC, WB, IP, IHC-P, Flow Cyt more details
- Species reactivityReacts with: Human
Does not react with
Mouse, Rat - Immunogen
A synthetic peptide corresponding to residues in Human Apg12.
- Positive controlTHP1, HepG2, Raji, and Human fetal kidney lysate; Apg12-transfected 293T lysates; Human colon tissue.
- General notesProduced under U.S. Patent No. 5,675,063.
Properties
- FormLiquid
- Storage instructionsStore at -20°C. Stable for 12 months at -20°C
- Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
- PurityTissue culture supernatant
- Clonality Monoclonal
- Clone numberEPR4799
- IsotypeIgG
-
Research Areas
Applications
Our Abpromise guarantee covers the use of ab109492 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ICC | ICC: 1/100 - 1/250. |
| WB | WB: 1/1000 - 1/10000. Predicted molecular weight: 15 kDa. |
| IP | IP: 1/10 - 1/100. |
| IHC-P | IHC-P: 1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
| Flow Cyt | Flow Cyt: 1/10 - 1/100. |
Target
- FunctionUbiquitin-like protein required for autophagy. Conjugated to ATG3 and ATG5.
- Tissue specificityUbiquitous.
- Sequence similaritiesBelongs to the ATG12 family.
- DomainShares weak sequence similarity with ubiquitin family, but contains an 'ubiquitin superfold' and the C-terminal Gly is required for isopeptide linkage.
- Cellular localizationCytoplasm.
-
Database links
- Entrez Gene: 9140 Human
- Entrez Gene: 9140 Human
- Entrez Gene: 9140 Human
- Omim: 609608 Human
- SwissProt: O94817 Human
- SwissProt: O94817 Human
- SwissProt: O94817 Human
- Unigene: 264482 Human
Target information above from: UniProt accession
O94817
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
.
-
Alternative names
- APG12-like antibodyAPG12L antibodyATG12 antibody
- ATG12 autophagy related 12 homolog (S. cerevisiae) antibodyATG12 autophagy related 12 homolog antibodyATG12_HUMAN antibodyAutophagy 12 antibodyAutophagy-related protein 12 antibodyFBR93 antibodyHAPG12 antibodyUbiquitin-like protein ATG12 antibody
see all
Anti-Apg12 antibody [EPR4799] images
-
![Western blot - Apg12 antibody [EPR4799] (ab109492)](http://a.abcam.com/ps/datasheet/images/109/ab109492/Apg12-Primary-antibodies-ab109492-1.jpg)
Western blot - Apg12 antibody [EPR4799] (ab109492)All lanes : Anti-Apg12 antibody [EPR4799] (ab109492) at 1/1000 dilution
Lane 1 : THP1 lysate
Lane 2 : HepG2 lysate
Lane 3 : Raji lysate
Lane 4 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Predicted band size : 15 kDa -
![Western blot - Apg12 antibody [EPR4799] (ab109492)](http://a.abcam.com/ps/datasheet/images/109/ab109492/Apg12-Primary-antibodies-ab109492-2.jpg)
Western blot - Apg12 antibody [EPR4799] (ab109492)All lanes : Anti-Apg12 antibody [EPR4799] (ab109492) at 1/1000 dilution
Lane 1 : Apg12-transfected 293T lysate
Lane 2 : Non-transfected 293T lysate
Lysates/proteins at 10 µg per lane.
Predicted band size : 15 kDa
Observed band size : 22 kDa (why is the actual band size different from the predicted?) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Apg12 antibody [EPR4799] (ab109492)](http://a.abcam.com/ps/datasheet/images/109/ab109492/Apg12-Primary-antibodies-ab109492-3.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Apg12 antibody [EPR4799] (ab109492)ab109492 at 1/50 dilution staining Apg12 in paraffin embedded Human colon tissue.
References for Anti-Apg12 antibody [EPR4799] (ab109492)
ab109492 has not yet been referenced specifically in any publications.
