|
|
|
|
Western blot - Apg12 antibody (ab4752)
(enlarge)
|
|
|
|
|
Product Name
|
Apg12 antibody
|
| |
See all Apg12 antibodies (9)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to Apg12
|
|
Immunogen
|
Recombinant full length protein (S. cerevisiae).
|
|
Reacts with
(species key)
|
Sc
|
|
Tested applications
(see key)
|
ELISA, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
ELISA: 1/1000 - 1/5000.
WB: 1/2000. Detects a band of approximately 22.1 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or their conjugates). Other intrinsic bands are readily detectable in yeast lysates at lower antibody dilutions.
|
|
Positive control
(see definition)
|
Most yeast cell lysates can be used as a positive control without induction or stimulation.
|
|
Cellular localization
|
Cytoplasmic
|
|
Research areas
|
Cancer >> Cancer Metabolism >> Autophagy
Cardiovascular >> Heart >> Autophagy >> APG gene products
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ub-like Proteins
|
|
Relevance
|
Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin. Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg8 and Apg12. Proteins of the second class include parkin, RAD23 and DSK2, are designated ubiquitin-domain proteins (UDPs). These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other. In contrast to UBLs, UDPs are not conjugated to other proteins. In yeast, autophagy, the delivery of cytoplasmic components to the lysosome/vacuole for degradation, requires a ubiquitin-like protein conjugation system, in which Apg12 is covalently bound to Apg12-Apg5 and Apg16.
|
|
|
|
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
IgG fraction
|
|
Storage buffer
|
Preservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Purification notes
|
Purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum.
|
|
Form
|
Liquid
|
|
Concentration
|
5.000 mg/ml
|
|
Storage instructions
|
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Apg12 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for Apg12 antibody images, references, products related to ab4752 and other tools.
|
| |
Apg12 antibody images:
|
 Western blot - Apg12 antibody (ab4752)
ab4752 generated by immunization with recombinant yeast APG12 was tested by immunoblot against yeast lysates expressing the APG12-GFP fusion protein and other UBL fusion proteins. All UBLs possess limited homology to Ubiquitin and to each other, therefore it is important to know the degree of reactivity of each antibody against each UBL. Panel A shows total protein staining using ponceau. Panel B shows positions of free GFP or GFP containing recombinant proteins present in each lysate preparation after reaction with a 1:1,000 dilution of Goat polyclonal to GFP (ab6673) followed by reaction with a 1:15,000 dilution of Donkey polyclonal to Goat IgG (HRP) (ab7125). Panel C shows specific reaction with APG12 using a 1:2,000 dilution of ab4752 followed by reaction with a 1:15,000 dilution of Goat polyclonal to Rabbit IgG (HRP) (ab7090). All primary antibodies were diluted in TTBS buffer supplemented with 5% non-fat milk and incubated with the membranes overnight at 4° C. Yeast lysate proteins were separated by SDS-PAGE using a 15% gel. This data indicates that anti-APG12 is highly specific and does not cross react with other UBLs. A chemiluminescence system was used for signal detection (Roche). Other detection systems will yield similar results. Data contributed by M. Malakhov, www.lifesensors.com, personal communication.
|
| |
References for Apg12 antibody (ab4752)
|
|
This product has been used in:
Crews L et al. Selective Molecular Alterations in the Autophagy Pathway in Patients with Lewy Body Disease and in Models of alpha-Synucleinopathy. PLoS One 5:e9313 (2010). WB; Human, Mouse. PubMed: 20174468
If you publish research using ab4752 please let us know so that we can cite the reference on this datasheet.
|
| |
|
Search PubMed (MEDLINE) for references to Apg12
|
| |
Apg12 antibody - more information
|
Customer reviews (feedback) regarding Apg12 antibody |
Customer FAQs regarding Apg12 antibody |
Protocols for Apg12 antibody |
Price and availability of products related to Apg12 antibody |
| |
Apg12 antibody - related products:
|
|
|
| |
Apg12 antibody - other tools:
|
Contact Abcam with a Technical Enquiry about Apg12 antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
