Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ub-like Proteins
Overview
Product name
Anti-Apg12 antibody
See all Apg12 products (11) ...
Description
Mouse monoclonal to Apg12
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Recombinant full length protein: MAEEPQSVLQ LPTSIAAGGE GLTDVSPETT TPEPPSSAAV SPGTEEPAGD TKKKIYLCES VLCSFPRPRS WNSL, corresponding to amino acids 1-75 of Human Apg12
MAEEPQSVLQ LPTSIAAGGE GLTDVSPETT TPEPPSSAAV SPGTEEPAGD TKKKIYLCES VLCSFPRPRS WNSL
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG1
Light chain type
kappa
Research areas
Cancer >> Signal transduction >> Autophagy
Cardiovascular >> Heart >> Autophagy >> APG gene products
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ub-like Proteins
-
Western blot - Apg12 antibody (ab56465)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab56465 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: Use at a concentration of 1-5 µg/ml.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Ubiquitin-like protein required for autophagy. Conjugated to ATG3 and ATG5.
Tissue specificity
Ubiquitous.
Sequence similarities
Belongs to the ATG12 family.
Domain
Shares weak sequence similarity with ubiquitin family, but contains an 'ubiquitin superfold' and the C-terminal Gly is required for isopeptide linkage.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionO94817
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- APG12-like antibody
- APG12L antibody
- ATG12 antibody
see all
Database links
- Entrez Gene: 9140 Human
- Omim: 609608 Human
- SwissProt: O94817 Human
- Unigene: 264482 Human
Anti-Apg12 antibody images:
Western blot - Apg12 antibody (ab56465)
Western blot against tagged recombinant protein immunogen using ab56465 Apg12 antibody at 1ug/ml. Predicted band size of immunogen is 34 kDa
References for Anti-Apg12 antibody (ab56465)
ab56465 has not yet been referenced specifically in any publications.
Publishing research using ab56465? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"