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Anti-Apg12 antibody (ab64208)

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Overview

Product name

Anti-Apg12 antibody
See all Apg12 products (11) ...

Description

Sheep polyclonal to Apg12

Tested applications

WB, IHC-P, IHC-Frmore details

Cross reactivity

Reacts with

Mouse, Rat

Immunogen

Synthetic peptide (100% identity for Mouse and Rat), conjugated to an immunogenic carrier protein.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C (add glycerol to a final volume of 40% for extra stability). Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: PBS, Whole serum

Purity

Whole antiserum

Clonality

Polyclonal

Isotype

IgG

Research areas

Cancer >> Signal transduction >> Autophagy
Cardiovascular >> Heart >> Autophagy >> APG gene products
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ub-like Proteins

Applications

Show applications key

Our Abpromise guarantee covers the use of ab64208 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide

    WB

     WB: 1/300 - 1/3000.Predi...Read more →

    WB: 1/300 - 1/3000.Predicted molecular weight: 16 kDa.(This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.)

  • ShowHide

    IHC-P

     IHC-P: 1/300 - 1/3000.Pe...Read more →

    IHC-P: 1/300 - 1/3000.Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • IHC-Fr

     IHC-Fr: 1/300 - 1/3000.

    IHC-Fr: 1/300 - 1/3000.

Target

Function

Ubiquitin-like protein required for autophagy. Conjugated to ATG3 and ATG5.

Tissue specificity

Ubiquitous.

Sequence similarities

Belongs to the ATG12 family.

Domain

Shares weak sequence similarity with ubiquitin family, but contains an 'ubiquitin superfold' and the C-terminal Gly is required for isopeptide linkage.

Cellular localization

Cytoplasm.

Target information above from: UniProt accessionO94817 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • APG12-like antibody
  • APG12L antibody
  • ATG12 antibody
  • ATG12 autophagy related 12 homolog (S. cerevisiae) antibody
  • ATG12 autophagy related 12 homolog antibody
  • ATG12_HUMAN antibody
  • Autophagy 12 antibody
  • Autophagy-related protein 12 antibody
  • FBR93 antibody
  • HAPG12 antibody
  • Ubiquitin-like protein ATG12 antibody
see all

Database links

References for Anti-Apg12 antibody (ab64208)

ab64208 has not yet been referenced specifically in any publications.

Publishing research using ab64208? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"