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Anti-Apg12 antibody (ab64223)

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    Overview

    Product name

    Anti-Apg12 antibody
    See all Apg12 products (11) ...

    Description

    Rabbit polyclonal to Apg12

    Tested applications

    WB, IHC-P, IHC-Frmore details

    Cross reactivity

    Reacts with

    Mouse, Rat

    Immunogen

    A synthetic peptide as a part of rat and mouse Apg12 conjugated to an immunogenic carrier protein

    Properties

    Form

    Liquid

    Storage instructions

    Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C (add glycerol to a final volume of 40% for extra stability). Avoid repeated freeze / thaw cycles.

    Storage buffer

    Preservative: None
    Constituents: PBS, Whole serum

    Purity

    Whole antiserum

    Clonality

    Polyclonal

    Isotype

    IgG

    Research areas

    Cancer >> Signal transduction >> Autophagy
    Cardiovascular >> Heart >> Autophagy >> APG gene products
    Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
    Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ub-like Proteins

    Applications

    Show applications key

    Our Abpromise guarantee covers the use of ab64223 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • ShowHide1 Image

      WB

      No star rating (1 Abreview) WB: 1/300 - 1/2000.Predi...Read more →

      WB: 1/300 - 1/2000.Predicted molecular weight: 16 kDa.(This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.)

    • ShowHide

      IHC-P

       IHC-P: 1/300 - 1/2000.Pe...Read more →

      IHC-P: 1/300 - 1/2000.Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • IHC-Fr

       IHC-Fr: 1/300 - 1/2000.

      IHC-Fr: 1/300 - 1/2000.

    Target

    Function

    Ubiquitin-like protein required for autophagy. Conjugated to ATG3 and ATG5.

    Tissue specificity

    Ubiquitous.

    Sequence similarities

    Belongs to the ATG12 family.

    Domain

    Shares weak sequence similarity with ubiquitin family, but contains an 'ubiquitin superfold' and the C-terminal Gly is required for isopeptide linkage.

    Cellular localization

    Cytoplasm.

    Target information above from: UniProt accessionO94817 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010).

    Information by UniProt

    Alternative names

    • APG12-like antibody
    • APG12L antibody
    • ATG12 antibody
    • ATG12 autophagy related 12 homolog (S. cerevisiae) antibody
    • ATG12 autophagy related 12 homolog antibody
    • ATG12_HUMAN antibody
    • Autophagy 12 antibody
    • Autophagy-related protein 12 antibody
    • FBR93 antibody
    • HAPG12 antibody
    • Ubiquitin-like protein ATG12 antibody
    see all

    Database links

    References for Anti-Apg12 antibody (ab64223)

    ab64223 has not yet been referenced specifically in any publications.

    Publishing research using ab64223? Please let us know so that we can cite the reference in this datasheet

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"