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Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ub-like Proteins
Overview
Product name
Anti-Apg3 antibody
See all Apg3 products (8) ...
Description
Rabbit polyclonal to Apg3
Tested applications
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat, Sheep, Cow, Zebrafish, Rhesus monkey
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Human Apg3.
(Peptide available as ab66285.)
Positive control
This antibody gave a positive signal in the following Lysates: Mouse Liver Tissue, Jurkat Whole Cell, TE 671 Whole Cell, Mouse Kidney Tissue
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Signal transduction >> Autophagy
Cardiovascular >> Heart >> Autophagy >> APG gene products
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ub-like Proteins
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Western blot - Apg3 antibody (ab47815)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab47815 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
Target
Function
E2-like enzyme involved in autophagy and mitochondrial homeostasis. Catalyzes the conjugation of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A) to phosphatidylethanolamine (PE). PE-conjugation to ATG8-like proteins is essential for autophagy. Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3.
Tissue specificity
Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta.
Sequence similarities
Belongs to the ATG3 family.
Post-translational
modifications
Conjugated to ATG12 at Lys-243. ATG12-conjugation plays a role in regulation of mitochondrial homeostasis and cell death, while it is not involved in PE-conjugation to ATG8-like proteins and autophagy.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionQ9NT62
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 2610016C12Rik antibody
- Apg 3 antibody
- APG3 autophagy 3 like antibody
see all
Database links
- Entrez Gene: 64422 Human
- Entrez Gene: 67841 Mouse
- Entrez Gene: 171415 Rat
- Entrez Gene: 336632 Zebrafish
- Omim: 609606 Human
- SwissProt: Q9NT62 Human
- SwissProt: Q9CPX6 Mouse
- SwissProt: Q6AZ50 Rat
see all
Anti-Apg3 antibody images:
Western blot - Apg3 antibody (ab47815)
All lanes : Anti-Apg3 antibody (ab47815) at 1 µg/ml
Lane 1 : Liver (Mouse) Tissue Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
Lane 4 : Kidney (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 36 kDa
Observed band size : 36 kDa
Additional bands at : 20 kDa. We are unsure as to the identity of these extra bands.
References for Anti-Apg3 antibody (ab47815)
ab47815 has not yet been referenced specifically in any publications.
Publishing research using ab47815? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"