Apg3 antibody (ab47815)

Product Name 

Apg3 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Apg3

Immunogen 

Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Human Apg3.

(Peptide available as ab66285.)

Reacts with 

Hu, Ms

Predicted to react 
(species key)

Rat, Cow, RMk, Shp, Zfsh
(Due to sequence homology)

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

This antibody gave a positive signal in the following Lysates:
Mouse Liver Tissue, Jurkat Whole Cell, TE 671 Whole Cell, Mouse Kidney Tissue

Cellular localization 

Cytoplasmic

Research areas 

Cancer >> Cancer Metabolism >> Autophagy
Cardiovascular >> Heart >> Autophagy >> APG gene products
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ub-like Proteins

Relevance 

A major contributor to cellular homeostasis is the ability of the cell to strike a balance between the formation and degradation/removal of its cellular components. This process of internal cellular turn-over is called autophagy (self-eating), and is facilitated by a pathway of around 16 interacting proteins in the human. APG3 is a conjugating enzyme for APG8 lipidation, an essential step for the initiation of autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3. A disruption to the autophagic process is now associated with the progression of several cancers, neurodegenerative disorders and cardiac pathologies.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.200 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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