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Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ub-like Proteins
Overview
Product name
Anti-Apg3 antibody
See all Apg3 products (8) ...
Description
Mouse monoclonal to Apg3
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment: MQNVINTVKG KALEVAEYLT PVLKESKFKE TGVITPEEFV AAGDHLVHHC PTWQWATGEE LKVKAYLPTG KQFLVTKNVP CYKRCKQMEY SDELEAIIEE , corresponding to amino acids 1-101 of Human Apg3
MQNVINTVKG KALEVAEYLT PVLKESKFKE TGVITPEEFV AAGDHLVHHC PTWQWATGEE LKVKAYLPTG KQFLVTKNVP CYKRCKQMEY SDELEAIIEE
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG2b
Light chain type
kappa
Research areas
Cancer >> Signal transduction >> Autophagy
Cardiovascular >> Heart >> Autophagy >> APG gene products
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ub-like Proteins
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Western blot - Apg3 antibody (ab56409)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab56409 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: Use at a concentration of 1-5 µg/ml.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
E2-like enzyme involved in autophagy and mitochondrial homeostasis. Catalyzes the conjugation of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A) to phosphatidylethanolamine (PE). PE-conjugation to ATG8-like proteins is essential for autophagy. Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3.
Tissue specificity
Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta.
Sequence similarities
Belongs to the ATG3 family.
Post-translational
modifications
Conjugated to ATG12 at Lys-243. ATG12-conjugation plays a role in regulation of mitochondrial homeostasis and cell death, while it is not involved in PE-conjugation to ATG8-like proteins and autophagy.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionQ9NT62
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 2610016C12Rik antibody
- Apg 3 antibody
- APG3 autophagy 3 like antibody
see all
Database links
- Entrez Gene: 64422 Human
- Omim: 609606 Human
- SwissProt: Q9NT62 Human
- Unigene: 477126 Human
Anti-Apg3 antibody images:
Western blot - Apg3 antibody (ab56409)
Apg3 antibody (ab56409) at 1ug/lane + Jurkat cell lysate at 25ug/lane.
References for Anti-Apg3 antibody (ab56409)
ab56409 has not yet been referenced specifically in any publications.
Publishing research using ab56409? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"