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Western blot - Apg3 antibody (ab56409)
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Product Name
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Apg3 antibody
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See all Apg3 antibodies (6)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal to Apg3
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Immunogen
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Recombinant fragment: MQNVINTVKG KALEVAEYLT PVLKESKFKE TGVITPEEFV AAGDHLVHHC PTWQWATGEE LKVKAYLPTG KQFLVTKNVP CYKRCKQMEY SDELEAIIEE , corresponding to amino acids 1-101 of Human Apg3
BLAST 'MQNVINTVKG KALEVAEYLT PVLKESKFKE TGVITPEEFV AAGDHLVHHC PTWQWATGEE LKVKAYLPTG KQFLVTKNVP CYKRCKQMEY SDELEAIIEE ' with  or 
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Reacts with
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Hu
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Tested applications
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 1-5 µg/ml.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cytoplasmic
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Research areas
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Cancer >> Cancer Metabolism >> Autophagy
Cardiovascular >> Heart >> Autophagy >> APG gene products
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ub-like Proteins
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Relevance
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A major contributor to cellular homeostasis is the ability of the cell to strike a balance between the formation and degradation/removal of its cellular components. This process of internal cellular turn-over is called autophagy (self-eating), and is facilitated by a pathway of around 16 interacting proteins in the human. APG3 is a conjugating enzyme for APG8 lipidation, an essential step for the initiation of autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3. A disruption to the autophagic process is now associated with the progression of several cancers, neurodegenerative disorders and cardiac pathologies.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Isotype
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IgG2b
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Light chain type
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kappa
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Purity
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Protein G purified
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Storage buffer
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Preservative: None
PBS, pH 7.2
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Apg3 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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Apg3 antibody images:
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 Western blot - Apg3 antibody (ab56409)
Predicted band size : 36 kDa
Apg3 antibody (ab56409) at 1ug/lane + Jurkat cell lysate at 25ug/lane.
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Search PubMed (MEDLINE) for references to Apg3
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Apg3 antibody - more information
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Customer reviews (feedback) regarding Apg3 antibody |
Customer FAQs regarding Apg3 antibody |
Protocols for Apg3 antibody |
Price and availability of products related to Apg3 antibody |
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Apg3 antibody - other tools:
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Contact Abcam with a Technical Enquiry about Apg3 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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