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Anti-Apg3 antibody (ab56409)

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Overview

Product name

Anti-Apg3 antibody
See all Apg3 products (8) ...

Description

Mouse monoclonal to Apg3

Tested applications

WBmore details

Cross reactivity

Reacts with

Human

Immunogen

Recombinant fragment: MQNVINTVKG KALEVAEYLT PVLKESKFKE TGVITPEEFV AAGDHLVHHC PTWQWATGEE LKVKAYLPTG KQFLVTKNVP CYKRCKQMEY SDELEAIIEE , corresponding to amino acids 1-101 of Human Apg3

MQNVINTVKG KALEVAEYLT PVLKESKFKE TGVITPEEFV AAGDHLVHHC PTWQWATGEE LKVKAYLPTG KQFLVTKNVP CYKRCKQMEY SDELEAIIEE

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
PBS, pH 7.2

Concentration

Concentration information loading...

Purity

Protein G purified

Clonality

Monoclonal

Isotype

IgG2b

Light chain type

kappa

  • Western blot - Apg3 antibody (ab56409)Western blot - Apg3 antibody (ab56409) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab56409 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

WB: Use at a concentration of 1-5 µg/ml.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

E2-like enzyme involved in autophagy and mitochondrial homeostasis. Catalyzes the conjugation of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A) to phosphatidylethanolamine (PE). PE-conjugation to ATG8-like proteins is essential for autophagy. Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3.

Tissue specificity

Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta.

Sequence similarities

Belongs to the ATG3 family.

Post-translational
modifications

Conjugated to ATG12 at Lys-243. ATG12-conjugation plays a role in regulation of mitochondrial homeostasis and cell death, while it is not involved in PE-conjugation to ATG8-like proteins and autophagy.

Cellular localization

Cytoplasm.

Target information above from: UniProt accessionQ9NT62 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • 2610016C12Rik antibody
  • Apg 3 antibody
  • APG3 autophagy 3 like antibody
  • APG3 like antibody
  • APG3-like antibody
  • APG3L antibody
  • Apg3p antibody
  • ATG 3 antibody
  • ATG3 antibody
  • ATG3 autophagy related 3 homolog antibody
  • ATG3 autophagy related 3 homolog (S. cerevisiae) antibody
  • ATG3_HUMAN antibody
  • Autophagy Apg3p/Aut1p like antibody
  • Autophagy related protein 3 antibody
  • Autophagy-related protein 3 antibody
  • DKFZp564M1178 antibody
  • FLJ22125 antibody
  • hApg3 antibody
  • MGC15201 antibody
  • OTTHUMP00000214547 antibody
  • OTTHUMP00000214548 antibody
  • PC3 96 antibody
  • Protein PC3-96 antibody
  • Ubiquitin-like-conjugating enzyme ATG3 antibody
see all

Anti-Apg3 antibody images:

  Western blot - Apg3 antibody (ab56409)

Western blot - Apg3 antibody (ab56409)

Apg3 antibody (ab56409) at 1ug/lane + Jurkat cell lysate at 25ug/lane.

References for Anti-Apg3 antibody (ab56409)

ab56409 has not yet been referenced specifically in any publications.

Publishing research using ab56409? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"