Overview
- Product nameAnti-Apg3 antibodySee all Apg3 primary antibodies ...
- DescriptionSheep polyclonal to Apg3
- Specificityab62111 is specific for Apg3.
- Tested applicationsWB, IHC-P, IHC-Fr more details
- Species reactivityReacts with: Mouse, Rat, Human
- Immunogen
Synthetic peptide corresponding to a part of human, rat and mouse Apg3 conjugated to immunogenic carrier protein.
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50%, aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: PBS -
Concentration information loading... - PurityIgG fraction
- Clonality Polyclonal
- IsotypeIgG
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Research Areas
Applications
Our Abpromise guarantee covers the use of ab62111 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 35 kDa. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein. |
| IHC-P | IHC-P: Use a concentration of 1 - 10 µg/ml. |
| IHC-Fr | IHC-Fr: Use a concentration of 1 - 10 µg/ml. |
Target
- FunctionE2-like enzyme involved in autophagy and mitochondrial homeostasis. Catalyzes the conjugation of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A) to phosphatidylethanolamine (PE). PE-conjugation to ATG8-like proteins is essential for autophagy. Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3.
- Tissue specificityWidely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta.
- Sequence similaritiesBelongs to the ATG3 family.
- Post-translational
modificationsConjugated to ATG12 at Lys-243. ATG12-conjugation plays a role in regulation of mitochondrial homeostasis and cell death, while it is not involved in PE-conjugation to ATG8-like proteins and autophagy. - Cellular localizationCytoplasm.
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Database links
- Entrez Gene: 64422 Human
- Entrez Gene: 64422 Human
- Entrez Gene: 67841 Mouse
- Entrez Gene: 171415 Rat
- Omim: 609606 Human
- SwissProt: Q9NT62 Human
- SwissProt: Q9NT62 Human
- SwissProt: Q9CPX6 Mouse
- SwissProt: Q6AZ50 Rat
- Unigene: 477126 Human
- Unigene: 41775 Mouse
- Unigene: 3084 Rat
see all
Target information above from: UniProt accession
Q9NT62
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- 2610016C12Rik antibodyApg 3 antibodyAPG3 antibody
- APG3 autophagy 3 like antibodyAPG3 like antibodyAPG3-like antibodyAPG3L antibodyApg3p antibodyATG 3 antibodyATG3 antibodyATG3 autophagy related 3 homolog antibodyATG3 autophagy related 3 homolog (S. cerevisiae) antibodyATG3_HUMAN antibodyAutophagy Apg3p/Aut1p like antibodyAutophagy related protein 3 antibodyAutophagy-related protein 3 antibodyDKFZp564M1178 antibodyFLJ22125 antibodyhApg3 antibodyMGC15201 antibodyOTTHUMP00000214547 antibodyOTTHUMP00000214548 antibodyPC3 96 antibodyProtein PC3-96 antibodyUbiquitin-like-conjugating enzyme ATG3 antibody
see all
References for Anti-Apg3 antibody (ab62111)
ab62111 has not yet been referenced specifically in any publications.
