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Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ub-like Proteins
Overview
Product name
Anti-Apg3 antibody
See all Apg3 products (8) ...
Description
Rabbit polyclonal to Apg3
Tested applications
WB, ELISA, IHC-Frmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Dog, Saccharomyces cerevisiae, Monkey
Immunogen
Synthetic peptide corresponding to a central region of human Apg3
Properties
Form
Lyophilised:
Add 100 ul sterile water and centrifuge to remove any insoluble material.
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C (add glycerol to a final volume of 50% for extra stability). Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: Whole serum
Concentration
Concentration information loading...
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Signal transduction >> Autophagy
Cardiovascular >> Heart >> Autophagy >> APG gene products
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ub-like Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab72003 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/100 - 1/1000.Predicted molecular weight: 35 kDa.
ELISA
ELISA: 1/5000ELISA: 1/5000
IHC-Fr: Use at an assay dependent dilution.
Target
Function
E2-like enzyme involved in autophagy and mitochondrial homeostasis. Catalyzes the conjugation of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A) to phosphatidylethanolamine (PE). PE-conjugation to ATG8-like proteins is essential for autophagy. Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3.
Tissue specificity
Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta.
Sequence similarities
Belongs to the ATG3 family.
Post-translational
modifications
Conjugated to ATG12 at Lys-243. ATG12-conjugation plays a role in regulation of mitochondrial homeostasis and cell death, while it is not involved in PE-conjugation to ATG8-like proteins and autophagy.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionQ9NT62
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 2610016C12Rik antibody
- Apg 3 antibody
- APG3 autophagy 3 like antibody
see all
Database links
- Entrez Gene: 64422 Human
- Entrez Gene: 67841 Mouse
- Entrez Gene: 171415 Rat
- Omim: 609606 Human
- SwissProt: Q9NT62 Human
- SwissProt: Q9CPX6 Mouse
- SwissProt: Q6AZ50 Rat
- Unigene: 477126 Human
see all
References for Anti-Apg3 antibody (ab72003)
ab72003 has not yet been referenced specifically in any publications.
Publishing research using ab72003? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"