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Apg7 293T Transfected Lysate - (positive control) (ab94329)

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Overview

  • Product nameApg7 293T Transfected Lysate - (positive control)
  • General notesab94329 is a 293T cell transfected lysate in which Human Apg7 has been transiently over-expressed using a pCMV-Apg7 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsWB more details

Properties

  • BackgroundDomain: The C-terminal part of the protein is essential for the dimerization and interaction with ATG3 and ATG12. Function: Functions as an E1 enzyme essential for multisubstrates such as ATG8-like proteins and ATG12. Forms intermediate conjugates with ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). PE-conjugation to ATG8-like proteins is essential for autophagy. Also acts as an E1 enzyme for ATG12 conjugation to ATG5 and ATG3. Similarity: Belongs to the ATG7 family. Tissue specificity: Widely expressed, especially in kidney, liver, lymph nodes and bone marrow.

    • Applications

    Our Abpromise guarantee covers the use of ab94329 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB
  • Application notesWB: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Apg7 293T Transfected Lysate - (positive control) images

    • SDS-PAGE - Apg7 293T Transfected Lysate - (positive control) (ab94329)
      SDS-PAGE - Apg7 293T Transfected Lysate - (positive control) (ab94329)
      ab94329 at 15µg/lane on an SDS-PAGE gel.
    • Western blot - Apg7 293T Transfected Lysate - (positive control) (ab94329)
      Western blot - Apg7 293T Transfected Lysate - (positive control) (ab94329)
      All lanes : Anti-Apg7 antibody (ab89775) at 1/500 dilution

      Lane 1 : Apg7 293T Transfected Lysate - (positive control) (ab94329)
      Lane 2 : 293T non-transfected lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

    References for Apg7 293T Transfected Lysate - (positive control) (ab94329)

    ab94329 has not yet been referenced specifically in any publications.

    Publishing research using ab94329 ? Please let us know so that we can cite the reference in this datasheet

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"