Overview

  • Product nameAnti-Apg7 antibodySee all Apg7 primary antibodies ...
  • Description
    Rabbit polyclonal to Apg7
  • Tested applicationsIHC-FoFr, ICC/IF, WB, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Chicken, Human
  • Immunogen

    A 17 amino acid synthetic peptide from near the C-terminus of human APG7.

  • Positive control
    • Caco-2 cell lysate, MCF7 cells.

Applications

Our Abpromise guarantee covers the use of ab53255 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration. PubMed: 21784844
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa).
IHC-P Use at an assay dependent dilution. PubMed: 20174468
IHC-Fr Use at an assay dependent concentration.

Target

  • FunctionFunctions as an E1 enzyme essential for multisubstrates such as ATG8-like proteins and ATG12. Forms intermediate conjugates with ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). PE-conjugation to ATG8-like proteins is essential for autophagy. Also acts as an E1 enzyme for ATG12 conjugation to ATG5 and ATG3.
  • Tissue specificityWidely expressed, especially in kidney, liver, lymph nodes and bone marrow.
  • Sequence similaritiesBelongs to the ATG7 family.
  • DomainThe C-terminal part of the protein is essential for the dimerization and interaction with ATG3 and ATG12.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1810013K23Rik antibody
    • Apg 7 antibody
    • APG7 autophagy 7 like antibody
    • APG7 autophagy 7-like (S. cerevisiae) antibody
    • APG7 like antibody
    • APG7, S. cerevisiae, homolog of antibody
    • APG7-like antibody
    • APG7L antibody
    • ATG 7 antibody
    • ATG12-activating enzyme E1 ATG7 antibody
    • atg7 antibody
    • ATG7 autophagy related 7 homolog (S. cerevisiae) antibody
    • ATG7 autophagy related 7 homolog antibody
    • ATG7_HUMAN antibody
    • Atg7l antibody
    • Autophagy 7, S. cerevisiae, homolog of antibody
    • Autophagy related protein 7 antibody
    • Autophagy-related 7 (yeast) antibody
    • Autophagy-related protein 7 antibody
    • DKFZp434N0735 antibody
    • GSA 7 antibody
    • GSA7 antibody
    • hAGP7 antibody
    • Ubiquitin activating enzyme E1 like protein antibody
    • Ubiquitin-activating enzyme E1-like protein antibody
    • Ubiquitin-like modifier-activating enzyme ATG7 antibody
    see all

Anti-Apg7 antibody images

  • Lane 1 : Anti-Apg7 antibody (ab53255) at 0.5 µg/ml
    Lane 2 : Anti-Apg7 antibody (ab53255) at 1 µg/ml
    Lane 3 : Anti-Apg7 antibody (ab53255) at 2 µg/ml

    Lane 1 : Caco-2 cell lysate at 15 µg per lane.
    Lane 2 : Caco-2 cell lysate at 15 µg per lane.
    Lane 3 : Caco-2 cell lysate at 15 µg per lane.


    Predicted band size : 78 kDa
    Observed band size : 78 kDa
  • This image shows ab53255 staining MCF7 cells as 10µg/ml
  • IHC image of ab53255 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab53255, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunohistochemical analysis of murine brain tissue, staining Apg7 with ab53255. Fixed brain sections were blocked with serum, permeabilized with 0.2% Triton X-100, and treated with primary antibody. An AlexaFluor®555-conjugated anti-rabbit IgG was used as the secondary antibody.

References for Anti-Apg7 antibody (ab53255)

This product has been referenced in:
  • Zhang H  et al. ROS-Mediated Autophagy Induced by Dysregulation of Lipid Metabolism Plays a Protective Role in Colorectal Cancer Cells Treated with Gambogic Acid. PLoS One 9:e96418 (2014). WB . Read more (PubMed: 24810758) »
  • Basit F  et al. Obatoclax (GX15-070) triggers necroptosis by promoting the assembly of the necrosome on autophagosomal membranes. Cell Death Differ 20:1161-73 (2013). Read more (PubMed: 23744296) »

See all 12 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Sample Chicken Cell (Retinal Ganglion Cell culture)
Specification Retinal Ganglion Cell culture
Permeabilization Yes - PBS + 0.1% Tween20
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Mar 10 2014

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
Sample Mouse Tissue sections (Uterus)
Specification Uterus
Permeabilization No
Fixative Paraformaldehyde
Username

Dr. Mukesh Jaiswal

Verified customer

Submitted Nov 13 2013

Thank you for confirming the PO number and for having provided us with your protocol.The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for th...

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Thank you for contacting us and for providing us with extra information about the protocol used.

All our products are covered by the Abpromise guarentee and since our product did not work as stated on the datasheet, I would be happy to replac...

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Thank you for taking time to contact us.

I am sorry to hear that this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for monit...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (THP-1 monocyte/macrophage cell line)
Specification THP-1 monocyte/macrophage cell line
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton X100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Aug 07 2012

Thank you very much for your interest in ab53255.

To our knowledge, ab53255 has not been tested in FACS. I can recommend ab52472 which has been tested if you are using human samples. It does not react with mouse or rat. Alternatively, I can ...

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Thank you for contacting us.

Your credit note ID is 20XXX (for 1 vial of ab91526).

I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The c...

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Thank you for providing that information.

Having reviewed the protocol used, I believe there are afew things that maybe worthtrying in order toimprove the specificity observed.I would suggest using a reducing agent in the loading buffer to re...

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Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly. The details pr...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"