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Overview

  • Product nameAnti-ApoER2 antibodySee all ApoER2 primary antibodies ...
  • Description
    Rabbit polyclonal to ApoER2
  • Conjugation notesKLH
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow, Monkey
  • Immunogen

    Synthetic peptide corresponding to amino acids 928-945 (Sequence GEPRSQLHQLPKNPLSEL) located near the C-terminus of human ApoER2, conjugated to KLH.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive controlHEK-293T cells expressing human ApoER2.

Properties

Applications

Our Abpromise guarantee covers the use of ab62659 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 110 kDa.

Target

  • FunctionCell surface receptor for Reelin (RELN) and apolipoprotein E (apoE)-containing ligands. LRP8 participates in transmitting the extracellular Reelin signal to intracellular signaling processes, by binding to DAB1 on its cytoplasmic tail. Reelin acts via both the VLDL receptor (VLDLR) and LRP8 to regulate DAB1 tyrosine phosphorylation and microtubule function in neurons. LRP8 has higher affinity for Reelin than VLDLR. LRP8 is thus a key component of the Reelin pathway which governs neuronal layering of the forebrain during embryonic brain development. Binds the endoplasmic reticulum resident receptor-associated protein (RAP). Binds dimers of beta 2-glycoprotein I and may be involved in the suppression of platelet aggregation in the vasculature. Highly expressed in the initial segment of the epididymis, where it affects the functional expression of clusterin and phospholipid hydroperoxide glutathione peroxidase (PHGPx), two proteins required for sperm maturation. May also function as an endocytic receptor.
  • Tissue specificityExpressed mainly in brain and placenta. Also expressed in platelets and megakaryocytic cells. Not expressed in the liver.
  • Involvement in diseaseDefects in LRP8 are a cause of myocardial infarction type 1 (MCI1) [MIM:608446]. A condition defined by the irreversible necrosis of heart muscle secondary to prolonged ischemia.
  • Sequence similaritiesBelongs to the LDLR family.
    Contains 2 EGF-like domains.
    Contains 7 LDL-receptor class A domains.
    Contains 5 LDL-receptor class B repeats.
  • DomainThe cytoplasmic domain is involved in the binding of DAB1 and in the recruitment of JNK-interacting proteins. Isoforms, which lack part of the cytoplasmic domain, are unable to recruit members of the family of JNK interacting proteins (JIP) to the cytoplasmic tail.
  • Post-translational
    modifications    O-glycosylated. Some alternatively spliced isoforms lack the O-linked sugar domain.
    Undergoes sequential, furin and gamma-secretase dependent, proteolytic processing, resulting in the extracellular release of the entire ligand-binding domain as a soluble polypeptide and in the intracellular domain (ICD) release into the cytoplasm. The gamma-secretase-dependent proteolytical processing occurs after the bulk of the extracellular domain has been shed, in a furin-dependent manner, in alternatively spliced isoforms carrying the furin cleavage site. Hypoglycosylation (mainly hypo-O-glycosylation) leads to increased extracellular cleavage, which in turn results in accelerating release of the intracellular domain (ICD) by the gamma-secretase. The resulting receptor fragment is able to inhibit Reelin signaling and in particular the Reelin-induced DAB1 phosphorylation.
    Tyrosine phosphorylated upon apoE binding.
    Ubiquitinated by MYLIP leading to degradation.
  • Cellular localizationCell membrane. Secreted. Isoforms that contain the exon coding for a furin-type cleavage site are proteolytically processed, leading to a secreted receptor fragment.

    • Target information above from: UniProt accession Q14114 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        APOER2 antibodyApolipoprotein E receptor 2 antibodylow density lipoprotein receptor-related protein 8 antibody
        Low-density lipoprotein receptor-related protein 8 antibodyLRP-8 antibodyLRP8 antibodyLRP8_HUMAN antibody
      see all

    Anti-ApoER2 antibody images

    • Western blot - ApoER2 antibody (ab62659)
      Western blot - ApoER2 antibody (ab62659)
      All lanes : Anti-ApoER2 antibody (ab62659) at 1/2000 dilution

      Lane 1 : HEK293 cells co-transfected with human ApoER2
      Lane 2 : wt HEK293 cells (untransfected)
      Lane 3 : Inhibition with ApoER2 (human 928-945) peptide (20 µg/mL)


      Predicted band size : 110 kDa

    References for Anti-ApoER2 antibody (ab62659)

    ab62659 has not yet been referenced specifically in any publications.

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