(MSA12)
ApoTrack™ Cytochrome c Apoptosis WB Antibody Cocktail (ab110415)
Overview
- Product nameApoTrack™ Cytochrome c Apoptosis WB Antibody CocktailSee all ApoTrack™ Cytochrome c Apoptosis kits ...
- Assay typeQuantitative
- Species reactivityReacts with: Human
- Product overview
The permeabilization of mitochondrial outer membrane and the subsequent release of cytochrome c and other apoptogenic proteins from mitochondrial intermembrane space into the cytoplasm is considered a hallmark of many apoptotic pathways. Therefore assaying these proteins in mitochondrial and cytoplasmic fractions is of prime interest for many researchers.
ab 110415 (MSA12) is a Western blot antibody cocktail that allows for the detection of cytochrome c in cytoplasmic and mitochondria-containing fractions for determining the proportion of released cytochrome c from mitochondria to the cytoplasm from apoptosis. The kit includes antibodies against a cytoplasmic protein marker, glyceraldehyde-3-phosphodehydrogenase (GAPDH), and 2 mitochondrial markers, pyruvate dehydrogenase subunit E1-alpha (a matrix marker), and ATP synthase subunit alpha (an inner membrane marker). This set of control markers allows for the monitoring and/or optimization of the permeabilization conditions.
Cocktail Antibodies:
Mouse anti Cyt. c monoclonal:
Amount: 50 µg
Working concentration: 1 µg/mlMouse anti GAPDH monclonal:
Amount: 5ug
Working concentration: 0.1 µg/mlMouse anti PDH-E1-alpha monoclonal:
Amount: 100ug
Working concentration: 2 µg/mlMouse anti C-V-alpha monoclonal:
Amount: 25ug
Working concentration: 0.5 µg/ml - Notes
Store cocktail at 4°C and control at -80°C
- Tested applicationsWB more details
Properties
- Storage instructionsStore at -80°C. Please refer to protocols.
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Components 180 µg ab110337 - Human Heart Mitochondria Control 1 unit Pre-mixed Solution of 4 monoclonal Antibodies 1 x 180µg - Research Areas
Applications
Our Abpromise guarantee covers the use of ab110415 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use a concentration of 3.6 µg/ml. The antibody cocktail (0.9 mg/ml) should be diluted 250x to a final working concentration of 3.6 µg/ml for Western blotting. |
ApoTrack™ Cytochrome c Apoptosis WB Antibody Cocktail images
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Western blot - ApoTrack™ Cytochrome c Apoptosis WB Antibody Cocktail (ab110415)In this experiment, apoptosis was induced in Jurkat and 143B osteosarcoma cells by FAS and also by treatment with staurosporine (HeLa cells were also treated, but only with STS). Mitochondrial and cytoplasmic fractions were isolated (using kit Cell Fractionation Kit ab109719/MS861) and probed using ab110415 (MSA12). As is clear from the gels, cytochrome c has translocated partially in FAS-induced cells and STS-treated osteosarcoma cells, and almost completely in STS-treated Jurkat and HeLa cells. The three control targets allow for verification of the "cleanness" of the cell fractionation.
References for ApoTrack™ Cytochrome c Apoptosis WB Antibody Cocktail (ab110415)
This product has been referenced in:
- Alquezar C et al. Inactivation of CDK/pRb pathway normalizes survival pattern of lymphoblasts expressing the FTLD-progranulin mutation c.709-1G>A. PLoS One 7:e37057 (2012). Read more (PubMed: 22623979) »
- Deas E et al. PINK1 cleavage at position A103 by the mitochondrial protease PARL. Hum Mol Genet 20:867-79 (2011). WB . Read more (PubMed: 21138942) »
