Apolipoprotein A I Human ELISA Kit (ab108803)
Overview
- Product nameApolipoprotein A I Human ELISA KitSee all Apolipoprotein A I kits ...
- Detection methodColorimetric
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Saliva, Milk, Urine, Cell culture extracts
- Assay typeSandwich
- Sensitivity= 3 ng/ml
- Range3.13 ng/ml - 200 ng/ml
- Recovery
94 %
- Assay durationMultiple steps standard assay
- Species reactivityReacts with: Human
- Product overview
Human Apolipoprotein A I (Apo A I) comprises about 70% of the high-density lipoproteins (HDL) protein mass and Apo A-II another 15–20%. Apolipoprotein A I, a 243-amino acid molecule that contains a series of highly homologous amphipathic alpha-helices, is a 28-kDa single polypeptide that lacks glycosylation or disulfide linkages. About 5–10% of Human plasma Apolipoprotein A I exists in a lipoprotein-unassociated state. Apolipoprotein A I appears to have effects on the atherosclerosis inhibition, reverse cholesterol transport and anti-inflammation.
ab108803 Apolipoprotein A I Human ELISA ((Enzyme-Linked Immunosorbent Assay) kit is designed for detection of Human Apolipoprotein A I in urine, saliva, milk, and cell culture supernatant. This assay employs a quantitative sandwich enzyme immunoassay technique that measures Human Apolipoprotein A I in less than 4 hours. A polyclonal antibody specific for Human Apolipoprotein A I has been pre-coated onto a 96-well microplate with removable strips. Apolipoprotein A I in standards and samples is sandwiched by the immobilized antibody and the biotinylated polyclonal antibody specific for Apolipoprotein A I, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The colour development is stopped and the intensity of the colour is measured.
- Tested applicationsELISA more details
- PlatformMicroplate
Properties
- Storage instructionsStore at +4°C. Please refer to protocols.
-
Components 1 x 96 tests Biotinylated Human Apolipoprotein A I Antibody (100x) 1 x 80µl Chromogen Substrate 1 x 8ml Diluent Concentrate (10x) 1 x 30ml Human Apolipoprotein A I Microplate: 96 wells 1 unit Human Apolipoprotein A I Standard 1 unit Stop Solution 1 x 12ml Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 80µl Wash Buffer Concentrate (20x) 2 x 30ml - Research Areas
-
Alternative names
- Apo-AIApoA-IApolipoprotein A-I
- Apolipoprotein A-I(1-242)Apolipoprotein A1
see all
- Entrez Gene: 335 Human
- Omim: 107680 Human
- SwissProt: P02647 Human
Applications
Our Abpromise guarantee covers the use of ab108803 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ELISA | ELISA |
Apolipoprotein A I Human ELISA Kit images
-
ELISA - Apolipoprotein A I Human ELISA Kit 1 x 96 well plate (ab108803)Standard curve
The curve provided is for illustration only. A standard curve should be generated each time the assay is performed.
Protocols
References for Apolipoprotein A I Human ELISA Kit (ab108803)
ab108803 has not yet been referenced specifically in any publications.
