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Purified mouse apolipoprotein AI from pooled mouse plasma high density lipoprotein.
Our Abpromise guarantee covers the use of ab20453 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|RID||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 31 kDa.|
|ELISA||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 19619511|
|Sandwich ELISA||Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Goat polyclonal to Apolipoprotein A I (ab7614).|
ab20453 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab20453 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.