Overview
- Product nameAnti-Apolipoprotein A I antibodySee all Apolipoprotein A I primary antibodies ...
- DescriptionRabbit polyclonal to Apolipoprotein A I
- Tested applicationsSandwich ELISA, WB, ICC more details
- Species reactivityReacts with: Mouse, Rat, Human
- Immunogen
Synthetic peptide: RLAEYHAKATEH, corresponding to amino acids 188 - 199 of Human Apolipoprotein A I
- Positive controlLiver, Intesine, HepG2 cell lysate.
Properties
- FormLiquid
- Storage instructionsStore at +4°C.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab33470 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Sandwich ELISA | sELISA: 1/2000 - 1/4000. Can be paired for Sandwich ELISA with Rabbit monoclonal [EP1368Y] to Apolipoprotein A I (ab52945). PubMed: 20421239 |
| WB | WB: Use a concentration of 1.5 µg/ml. Predicted molecular weight: 30 kDa. |
| ICC | ICC: Use a concentration of 4 µg/ml. |
Target
- FunctionParticipates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
- Tissue specificityMajor protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine.
- Involvement in diseaseDefects in APOA1 are a cause of high density lipoprotein deficiency type 2 (HDLD2) [MIM:604091]; also known as familial hypoalphalipoproteinemia (FHA). Inheritance is autosomal dominant.
Defects in APOA1 are a cause of the low HDL levels observed in high density lipoprotein deficiency type 1 (HDLD1) [MIM:205400]; also known as analphalipoproteinemia or Tangier disease (TGD). HDLD1 is a recessive disorder characterized by the absence of plasma HDL, accumulation of cholesteryl esters, premature coronary artery disease, hepatosplenomegaly, recurrent peripheral neuropathy and progressive muscle wasting and weakness. In HDLD1 patients, ApoA-I fails to associate with HDL probably because of the faulty conversion of pro-ApoA-I molecules into mature chains, either due to a defect in the converting enzyme activity or a specific structural defect in Tangier ApoA-I.
Defects in APOA1 are the cause of amyloid polyneuropathy-nephropathy Iowa type (AMYLIOWA) [MIM:107680]; also known as amyloidosis van Allen type or familial amyloid polyneuropathy type III. AMYLIOWA is a hereditary generalized amyloidosis due to deposition of amyloid mainly constituted by apolipoprotein A1. The clinical picture is dominated by neuropathy in the early stages of the disease and nephropathy late in the course. Death is due in most cases to renal amyloidosis. Severe peptic ulcer disease can occurr in some and hearing loss is frequent. Cataracts is present in several, but vitreous opacities are not observed.
Defects in APOA1 are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash. - Sequence similaritiesBelongs to the apolipoprotein A1/A4/E family.
- Post-translational
modificationsPalmitoylated.
Phosphorylation sites are present in the extracelllular medium. - Cellular localizationSecreted.
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Database links
- Entrez Gene: 335 Human
- Entrez Gene: 11806 Mouse
- Entrez Gene: 25081 Rat
- Omim: 107680 Human
- SwissProt: P02647 Human
- SwissProt: Q00623 Mouse
- SwissProt: P04639 Rat
- Unigene: 93194 Human
- Unigene: 26743 Mouse
- Unigene: 10308 Rat
see all
Target information above from: UniProt accession
P02647
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- Apo AI antibodyApo-AI antibodyApoA I antibody
- ApoA-I antibodyAPOA1 antibodyAPOA1/APOC3 fusion gene antibodyAPOA1_HUMAN antibodyApolipoprotein A I precursor antibodyApolipoprotein A-I(1-242) antibodyApolipoprotein A1 antibodyApolipoprotein AI antibodyApolipoprotein of high density lipoprotein antibodyApolipoproteinAI antibodyBrp14 antibodyLtw1 antibodyLvtw1 antibodyMGC117399 antibodySep1 antibodySep2 antibody
see all
References for Anti-Apolipoprotein A I antibody (ab33470)
This product has been referenced in:
- Meriwether D et al. Enhancement by LDL of transfer of L-4F and oxidized lipids to HDL in C57BL/6J mice and human plasma. J Lipid Res 52:1795-809 (2011). WB ; Human, Mouse . Read more (PubMed: 21804067) »
- Park KH et al. Senescence-related truncation and multimerization of apolipoprotein A-I in high-density lipoprotein with an elevated level of advanced glycated end products and cholesteryl ester transfer activity. J Gerontol A Biol Sci Med Sci 65:600-10 (2010). WB, Sandwich ELISA ; Human . Read more (PubMed: 20421239) »
