Overview
- Product nameAnti-Apolipoprotein A I antibodySee all Apolipoprotein A I primary antibodies ...
- DescriptionGoat polyclonal to Apolipoprotein A I
- SpecificityTypically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA. This antibody reacts with mouse apoLipoprotein A-I and has negligible cross-reactivity with Type A-II, B, C-I, C-II, C-III, E and J apoLipoproteins.
- Tested applicationsSandwich ELISA, Dot Blot, ELISA, IHC-P, WB more details
- Species reactivityReacts with: Mouse
- Immunogen
Full length native apoLipoprotein Type A-I (purified).
- General notesThis antibody has been used to determine that atherosclerotic lesions in the human aorta contain considerable amounts of lipoproteins. These lipoproteins were observed to be complexed with components of the extracellular matrix (especially LDL and proteoglycans). The role of these matrix-lipoprotein complexes is not entirely clear, however, animal models of atherosclerosis have shown that increased cellular proliferation and increased production of extracellular matrix components occur following injury to the intimal layer of the aorta.
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2 -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesThis product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities.
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab7614 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Sandwich ELISA | sELISA: Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Rabbit polyclonal to Apolipoprotein A I (ab20453). |
| Dot Blot | Dot: Use at an assay dependent dilution. |
| ELISA | ELISA: 1/4000 - 1/8000. |
| IHC-P | IHC-P: 1/50 - 1/200. |
| WB | WB: 1/5000 - 1/10000. |
Target
- FunctionParticipates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
- Tissue specificityMajor protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine.
- Involvement in diseaseDefects in APOA1 are a cause of high density lipoprotein deficiency type 2 (HDLD2) [MIM:604091]; also known as familial hypoalphalipoproteinemia (FHA). Inheritance is autosomal dominant.
Defects in APOA1 are a cause of the low HDL levels observed in high density lipoprotein deficiency type 1 (HDLD1) [MIM:205400]; also known as analphalipoproteinemia or Tangier disease (TGD). HDLD1 is a recessive disorder characterized by the absence of plasma HDL, accumulation of cholesteryl esters, premature coronary artery disease, hepatosplenomegaly, recurrent peripheral neuropathy and progressive muscle wasting and weakness. In HDLD1 patients, ApoA-I fails to associate with HDL probably because of the faulty conversion of pro-ApoA-I molecules into mature chains, either due to a defect in the converting enzyme activity or a specific structural defect in Tangier ApoA-I.
Defects in APOA1 are the cause of amyloid polyneuropathy-nephropathy Iowa type (AMYLIOWA) [MIM:107680]; also known as amyloidosis van Allen type or familial amyloid polyneuropathy type III. AMYLIOWA is a hereditary generalized amyloidosis due to deposition of amyloid mainly constituted by apolipoprotein A1. The clinical picture is dominated by neuropathy in the early stages of the disease and nephropathy late in the course. Death is due in most cases to renal amyloidosis. Severe peptic ulcer disease can occurr in some and hearing loss is frequent. Cataracts is present in several, but vitreous opacities are not observed.
Defects in APOA1 are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash. - Sequence similaritiesBelongs to the apolipoprotein A1/A4/E family.
- Post-translational
modificationsPalmitoylated.
Phosphorylation sites are present in the extracelllular medium. - Cellular localizationSecreted.
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Database links
- Entrez Gene: 11806 Mouse
- SwissProt: Q00623 Mouse
- Unigene: 26743 Mouse
Target information above from: UniProt accession
P02647
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- Apo AI antibodyApo-AI antibodyApoA I antibody
- ApoA-I antibodyAPOA1 antibodyAPOA1/APOC3 fusion gene antibodyAPOA1_HUMAN antibodyApolipoprotein A I precursor antibodyApolipoprotein A-I(1-242) antibodyApolipoprotein A1 antibodyApolipoprotein AI antibodyApolipoprotein of high density lipoprotein antibodyApolipoproteinAI antibodyBrp14 antibodyLtw1 antibodyLvtw1 antibodyMGC117399 antibodySep1 antibodySep2 antibody
see all
Anti-Apolipoprotein A I antibody images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Apolipoprotein A I antibody (ab7614)This image is a courtesy of Judit Marsillachab7614 staining Apolipoprotein A I in mouse liver tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before enzymatic antigen retrieval with Proteinase K and then blocking with 2% BSA for 20 minutes was performed. The primary antibody was diluted 1/6000 and incubated with sample for 24 hours at 4°C in 1% BSA. A Biotin conjugated goat polyclonal to rabbit IgG was used at dilution at 1/200 as secondary antibody.
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Western blot - Apolipoprotein A I antibody (ab7614)All lanes : Anti-Apolipoprotein A I antibody (ab7614) at 1/5000 dilution
Lane 1 : Whole tissue lysate prepared from mouse liver
Lane 2 : Whole tissue lysate prepared from mouse liver
Lane 3 : Whole tissue lysate prepared from mouse liver
Lysates/proteins at 50 µg per lane.
Secondary
Donkey anti-goat Alexa Fluor® 647 at 1/2500 dilution
Observed band size : 25 kDa (why is the actual band size different from the predicted?)
Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.Image courtesy of Hua Jiang by Abreview.
References for Anti-Apolipoprotein A I antibody (ab7614)
This product has been referenced in:
- Asano A et al. Characterization of the proteomes associating with three distinct membrane raft sub-types in murine sperm. Proteomics 10:3494-505 (2010). WB ; Mouse . Read more (PubMed: 20815087) »
- Shu X et al. Intravenous injection of apolipoprotein A-V reconstituted high-density lipoprotein decreases hypertriglyceridemia in apoav-/- mice and requires glycosylphosphatidylinositol-anchored high-density lipoprotein-binding protein 1. Arterioscler Thromb Vasc Biol 30:2504-9 (2010). WB ; Mouse . Read more (PubMed: 20966404) »
