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Full length native apoLipoprotein Type A-I (purified).
Our Abpromise guarantee covers the use of ab7614 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||1/5000 - 1/10000. Can be paired for Sandwich ELISA with Rabbit polyclonal to Apolipoprotein A I (ab20453).|
|ELISA||1/5000 - 1/10000.|
|IHC-P||1/250 - 1/500.|
|WB||1/500 - 1/1000.|
ab7614 staining Apolipoprotein A I in mouse liver tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before enzymatic antigen retrieval with Proteinase K and then blocking with 2% BSA for 20 minutes was performed. The primary antibody was diluted 1/6000 and incubated with sample for 24 hours at 4°C in 1% BSA. A Biotin conjugated goat polyclonal to rabbit IgG was used at dilution at 1/200 as secondary antibody.
Image courtesy of Hua Jiang by Abreview.