Overview

  • Product nameAnti-Apolipoprotein A I antibodySee all Apolipoprotein A I primary antibodies ...
  • Description
    Goat polyclonal to Apolipoprotein A I
  • SpecificityTypically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA. This antibody reacts with mouse apoLipoprotein A-I and has negligible cross-reactivity with Type A-II, B, C-I, C-II, C-III, E and J apoLipoproteins.
  • Tested applicationsSandwich ELISA, Dot Blot, ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Full length native apoLipoprotein Type A-I (purified).

  • General notesThis antibody has been used to determine that atherosclerotic lesions in the human aorta contain considerable amounts of lipoproteins. These lipoproteins were observed to be complexed with components of the extracellular matrix (especially LDL and proteoglycans). The role of these matrix-lipoprotein complexes is not entirely clear, however, animal models of atherosclerosis have shown that increased cellular proliferation and increased production of extracellular matrix components occur following injury to the intimal layer of the aorta.

Properties

Applications

Our Abpromise guarantee covers the use of ab7614 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Rabbit polyclonal to Apolipoprotein A I (ab20453).
Dot Blot Use at an assay dependent dilution.
ELISA 1/4000 - 1/8000.
IHC-P 1/50 - 1/200.
WB 1/5000 - 1/10000.

Target

  • FunctionParticipates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
  • Tissue specificityMajor protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine.
  • Involvement in diseaseDefects in APOA1 are a cause of high density lipoprotein deficiency type 2 (HDLD2) [MIM:604091]; also known as familial hypoalphalipoproteinemia (FHA). Inheritance is autosomal dominant.
    Defects in APOA1 are a cause of the low HDL levels observed in high density lipoprotein deficiency type 1 (HDLD1) [MIM:205400]; also known as analphalipoproteinemia or Tangier disease (TGD). HDLD1 is a recessive disorder characterized by the absence of plasma HDL, accumulation of cholesteryl esters, premature coronary artery disease, hepatosplenomegaly, recurrent peripheral neuropathy and progressive muscle wasting and weakness. In HDLD1 patients, ApoA-I fails to associate with HDL probably because of the faulty conversion of pro-ApoA-I molecules into mature chains, either due to a defect in the converting enzyme activity or a specific structural defect in Tangier ApoA-I.
    Defects in APOA1 are the cause of amyloid polyneuropathy-nephropathy Iowa type (AMYLIOWA) [MIM:107680]; also known as amyloidosis van Allen type or familial amyloid polyneuropathy type III. AMYLIOWA is a hereditary generalized amyloidosis due to deposition of amyloid mainly constituted by apolipoprotein A1. The clinical picture is dominated by neuropathy in the early stages of the disease and nephropathy late in the course. Death is due in most cases to renal amyloidosis. Severe peptic ulcer disease can occurr in some and hearing loss is frequent. Cataracts is present in several, but vitreous opacities are not observed.
    Defects in APOA1 are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash.
  • Sequence similaritiesBelongs to the apolipoprotein A1/A4/E family.
  • Post-translational
    modifications
    Palmitoylated.
    Phosphorylation sites are present in the extracelllular medium.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apo AI antibody
    • Apo-AI antibody
    • ApoA I antibody
    • ApoA-I antibody
    • APOA1 antibody
    • APOA1/APOC3 fusion gene antibody
    • APOA1_HUMAN antibody
    • Apolipoprotein A I precursor antibody
    • Apolipoprotein A-I(1-242) antibody
    • Apolipoprotein A1 antibody
    • Apolipoprotein AI antibody
    • Apolipoprotein of high density lipoprotein antibody
    • ApolipoproteinAI antibody
    • Brp14 antibody
    • Ltw1 antibody
    • Lvtw1 antibody
    • MGC117399 antibody
    • Sep1 antibody
    • Sep2 antibody
    see all

Anti-Apolipoprotein A I antibody images

  • ab7614 staining Apolipoprotein A I in mouse liver tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before enzymatic antigen retrieval with Proteinase K and then blocking with 2% BSA for 20 minutes was performed. The primary antibody was diluted 1/6000 and incubated with sample for 24 hours at 4°C in 1% BSA. A Biotin conjugated goat polyclonal to rabbit IgG was used at dilution at 1/200 as secondary antibody.

    See Abreview

  • All lanes : Anti-Apolipoprotein A I antibody (ab7614) at 1/5000 dilution

    Lane 1 : Whole tissue lysate prepared from mouse liver
    Lane 2 : Whole tissue lysate prepared from mouse liver
    Lane 3 : Whole tissue lysate prepared from mouse liver

    Lysates/proteins at 50 µg per lane.

    Secondary
    Donkey anti-goat Alexa Fluor® 647 at 1/2500 dilution

    Observed band size : 25 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.

    Image courtesy of Hua Jiang by Abreview.

References for Anti-Apolipoprotein A I antibody (ab7614)

This product has been referenced in:
  • Hesse D  et al. Hepatic trans-Golgi action coordinated by the GTPase ARFRP1 is crucial for lipoprotein lipidation and assembly. J Lipid Res 55:41-52 (2014). WB ; Mouse . Read more (PubMed: 24186947) »
  • Aseem O  et al. Cubilin Maintains Blood Levels of HDL and Albumin. J Am Soc Nephrol N/A:N/A (2013). Mouse . Read more (PubMed: 24357674) »

See all 8 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Liver)
Loading amount 10 µg
Specification Liver
Gel Running Conditions Reduced Non-Denaturing (Native) (4-20 %)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted Oct 02 2012

Thank you for contacting us. We currently only have a mouse ELISA kit for Apolipoprotein CIII: ab108811 However, we do have antibodies to each of your targets of interest that can be used in ELISA on mouse samples: Apolipoprotein A I: ab7614 or ab20453...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Liver, stomach, kidney, testes, lung, heart, etc)
Specification Liver, stomach, kidney, testes, lung, heart, etc
Fixative Formaldehyde
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: Proteinase K
Permeabilization No
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 2%
Username

Miss. Judit Marsillach

Verified customer

Submitted Jul 24 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (liver)
Loading amount 50 µg
Specification liver
Gel Running Conditions Reduced Denaturing (9%)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Hua Jiang

Verified customer

Submitted Jan 02 2009

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Human Purified protein (purified protein (BIODESIGN))
Loading amount 0.5 µg
Specification purified protein (BIODESIGN)
Gel Running Conditions Reduced Denaturing
Blocking step 5%mill+1%BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Feb 11 2008

Thank you for your enquiry. This product was tested using a direct ELISA using purified mouse Apo A-1. It was not tested using different concentrations of mouse serum. I am sorry you are experiencing problems with this antibody. Below please...

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Thank you for your enquiry and for taking your time to fill in the on-line Questionnaire. We are very sorry to hear that you are having problem with this antibody. We have searched our database and found that this is a popular selling product and y...

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Thank you for your enquiry. We would suggest performing a regular sandwich ELISA protocol.If you need anything further or any help then please let us know.

Thank you for your enquiry. The immunogen is from mouse that's why the antibody recognizes mouse Apolipoprotein A I.

Thank you for your enquiry. Unfortunately, the purified Apolipoprotein A I protein which has been used is not commercially available.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"