Anti-Apolipoprotein A II antibody [EPR2913] (ab92478)


  • Product nameAnti-Apolipoprotein A II antibody [EPR2913]
    See all Apolipoprotein A II primary antibodies
  • Description
    Rabbit monoclonal [EPR2913] to Apolipoprotein A II
  • Tested applicationsSuitable for: WB, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human Apolipoprotein A II.

  • Positive control
    • Human plasma lysate. HEPG2 cells; rat primary astrocytes,
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


Our Abpromise guarantee covers the use of ab92478 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).
Flow Cyt 1/50. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100 - 1/500.
  • Application notesIs unsuitable for IHC-P or IP.
  • Target

    • FunctionMay stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism.
    • Tissue specificityPlasma; synthesized in the liver and intestine.
    • Sequence similaritiesBelongs to the apolipoprotein A2 family.
    • Post-translational
      Phosphorylation sites are present in the extracelllular medium.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • APO A2 antibody
      • Apo AII antibody
      • Apo-AII antibody
      • APOA 2 antibody
      • ApoA II antibody
      • ApoA-II antibody
      • APOA2 antibody
      • APOA2_HUMAN antibody
      • APOAII antibody
      • Apolipoprotein A II antibody
      • Apolipoprotein A-II(1-76) antibody
      • Apolipoprotein A2 antibody
      • Apolipoprotein AII antibody
      • ApolipoproteinA II antibody
      • OTTHUMP00000032244 antibody
      see all

    Anti-Apolipoprotein A II antibody [EPR2913] images

    • Anti-Apolipoprotein A II antibody [EPR2913] (ab92478) at 1/1000 dilution + Human plasma lysate at 10 µg

      HRP labelled goat anti-rabbit antibody at 1/2000 dilution

      Predicted band size : 11 kDa
      Observed band size : 11 kDa
    • Immunocytochemistry/Immunofluorescence analysis rat primary astrocyte culture labelling Apolipoprotein A II with ab92478 at 1/500. Cells were fixed with methanol and permeabilised using 0.1M PBS with 1% Triton X. An Alexa Fluor® 488-conjugated monoclonal donkey antibody at 1/3000 dilution was used as the secondary antibody. 

      See Abreview

    • Ab92478 at 1/100 dilution staining Apolipoprotein A II in HepG2 cells by immunofluorescence.

    References for Anti-Apolipoprotein A II antibody [EPR2913] (ab92478)

    ab92478 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Rat Cell (Primary astrocyte culture)
    Permeabilization Yes - 0.1M PBS with 1% Triton X
    Specification Primary astrocyte culture
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
    Fixative Methanol

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted Sep 06 2016