Anti-Apolipoprotein A II antibody (ab24241)

Overview

• Product nameAnti-Apolipoprotein A II antibodySee all Apolipoprotein A II primary antibodies ...
• Description
  Rabbit polyclonal to Apolipoprotein A II
• Tested applicationsWB, ELISA, Conjugation, ICC/IF, IHC-P more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Purified human Apolipoprotein A II.

• Positive controlIF/ICC: HepG2 cell line. IHC-P: Liver FFPE tissue sections.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 75mM PBS, 75mM Sodium chloride, pH 7.2
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesHuman Apo AII Sepharose affinity column
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Types of disease
  • Heart disease

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Lipid and lipoprotein metabolism
  • Lipid metabolism

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Lipid and lipoprotein metabolism
  • Cholesterol Metabolism

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Energy transfer pathways
  • Energy Metabolism

  • Cancer
  • Cancer Metabolism
  • Metabolic signaling pathway
  • Metabolism of lipids and lipoproteins

  • Signal Transduction
  • Metabolism
  • Lipid metabolism

  • Signal Transduction
  • Metabolism
  • Energy Metabolism

  • Cardiovascular
  • Lipids / Lipoproteins
  • Lipoproteins/Apolipoproteins
  • Apolipoproteins

  • Cardiovascular
  • Atherosclerosis
  • Lipid transport

  • Cardiovascular
  • Lipids / Lipoproteins
  • Lipid Metabolism
  • Cholesterol Metabolism

Applications

Target

• FunctionMay stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism.
• Tissue specificityPlasma; synthesized in the liver and intestine.
• Sequence similaritiesBelongs to the apolipoprotein A2 family.
• Post-translational
  modificationsPhosphorylation sites are present in the extracelllular medium.
• Cellular localizationSecreted.

Target information above from: UniProt accession P02652 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 336 Human
  • Entrez Gene: 336 Human
  • Omim: 107670 Human
  • SwissProt: P02652 Human
  • SwissProt: P02652 Human
  • Unigene: 237658 Human

• Alternative names
  APO A2 antibodyApo AII antibodyApo-AII antibody

  APOA 2 antibodyApoA II antibodyApoA-II antibodyAPOA2 antibodyAPOA2_HUMAN antibodyAPOAII antibodyApolipoprotein A II antibodyApolipoprotein A-II(1-76) antibodyApolipoprotein A2 antibodyApolipoprotein AII antibodyApolipoproteinA II antibodyOTTHUMP00000032244 antibody

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Anti-Apolipoprotein A II antibody images

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein A II antibody (ab24241)
  ICC/IF image of ab24241 stained HepG2 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24241, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein A II antibody (ab24241)
  IHC image of Apolipoprotein A II staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab24241, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.