Overview

  • Product nameAnti-Apolipoprotein A II antibodySee all Apolipoprotein A II primary antibodies ...
  • Description
    Rabbit polyclonal to Apolipoprotein A II
  • Tested applicationsWB, ELISA, Conjugation, ICC/IF, IHC-P more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Purified human Apolipoprotein A II.

  • Positive control
    • IF/ICC: HepG2 cell line. IHC-P: Liver FFPE tissue sections.

Applications

Our Abpromise guarantee covers the use of ab24241 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/5000 - 1/40000. Predicted molecular weight: 11 kDa.
ELISA 1/5000 - 1/40000.
Conjugation Use at an assay dependent dilution.
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • FunctionMay stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism.
  • Tissue specificityPlasma; synthesized in the liver and intestine.
  • Sequence similaritiesBelongs to the apolipoprotein A2 family.
  • Post-translational
    modifications
    Phosphorylation sites are present in the extracelllular medium.
  • Cellular localizationSecreted.
  • Target information above from: UniProt accession P02652 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • APO A2 antibody
    • Apo AII antibody
    • Apo-AII antibody
    • APOA 2 antibody
    • ApoA II antibody
    • ApoA-II antibody
    • APOA2 antibody
    • APOA2_HUMAN antibody
    • APOAII antibody
    • Apolipoprotein A II antibody
    • Apolipoprotein A-II(1-76) antibody
    • Apolipoprotein A2 antibody
    • Apolipoprotein AII antibody
    • ApolipoproteinA II antibody
    • OTTHUMP00000032244 antibody
    see all

Anti-Apolipoprotein A II antibody images

  • ICC/IF image of ab24241 stained HepG2 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24241, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • IHC image of Apolipoprotein A II staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab24241, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-Apolipoprotein A II antibody (ab24241)

This product has been referenced in:
  • Tomazic PV  et al. Nasal mucus proteomic changes reflect altered immune responses and epithelial permeability in patients with allergic rhinitis. J Allergy Clin Immunol N/A:N/A (2013). WB ; Human . Read more (PubMed: 24290289) »

See 1 Publication for this product

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