Overview
- Product nameAnti-Apolipoprotein A II antibodySee all Apolipoprotein A II primary antibodies ...
- DescriptionMouse monoclonal to Apolipoprotein A II
- Tested applicationsIHC-P, WB more details
- Species reactivityReacts with: Human, Recombinant Fragment
- Immunogen
Recombinant full length protein: MKLLAATVLL LTICSLEGAL VRRQAKEPCV ESLVSQYFQT VTDYGKDLME KVKSPELQAE AKSYFEKSKE QLTPLIKKAG TELVNFLSYF VELGTQPATQ , corresponding to amino acids 1-101 of Human Apolipoprotein A II
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
PBS, pH 7.2 -
Concentration information loading... - PurityProtein G purified
- Clonality Monoclonal
- IsotypeIgG1
- Light chain typekappa
-
Research Areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
Applications
Our Abpromise guarantee covers the use of ab54796 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| IHC-P | IHC-P: Use a concentration of 3 µg/ml. |
| WB | WB: Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein. |
Target
- FunctionMay stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism.
- Tissue specificityPlasma; synthesized in the liver and intestine.
- Sequence similaritiesBelongs to the apolipoprotein A2 family.
- Post-translational
modificationsPhosphorylation sites are present in the extracelllular medium. - Cellular localizationSecreted.
-
Database links
- Entrez Gene: 336 Human
- Entrez Gene: 336 Human
- Omim: 107670 Human
- SwissProt: P02652 Human
- SwissProt: P02652 Human
- Unigene: 237658 Human
Target information above from: UniProt accession
P02652
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
.
-
Alternative names
- APO A2 antibodyApo AII antibodyApo-AII antibody
- APOA 2 antibodyApoA II antibodyApoA-II antibodyAPOA2 antibodyAPOA2_HUMAN antibodyAPOAII antibodyApolipoprotein A II antibodyApolipoprotein A-II(1-76) antibodyApolipoprotein A2 antibodyApolipoprotein AII antibodyApolipoproteinA II antibodyOTTHUMP00000032244 antibody
see all
Anti-Apolipoprotein A II antibody images
-
IHC-P - Apolipoprotein A II antibody (ab54796)Apolipoprotein A II antibody (ab54796) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human malignant lymphoma, diffuse large B. -
Western blot - Anti-Apolipoprotein A II antibody (ab54796)Western blot against tagged recombinant protein immunogen using ab54796 Apolipoprotein A II antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa -
Western blotAnti-Apolipoprotein A II antibody (ab54796) at 1 µg/ml +Anti-Apolipoprotein A II antibody (ab54796) at 0.1 µg
Secondary
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 16 minutes
References for Anti-Apolipoprotein A II antibody (ab54796)
ab54796 has not yet been referenced specifically in any publications.
