Overview
- Product nameApolipoprotein A1 Human ELISA KitSee all Apolipoprotein A I kits ...
- Detection methodColorimetric
- Tests1 x 96 well plate
- Sample typeSerum, Plasma
- Assay typeCompetitive
- Sensitivity1 µg/ml
- Range0.625 µg/ml - 20 µg/ml
- Recovery
96 %
- Assay time3h 00m
- Assay durationMultiple steps standard assay
- Species reactivityReacts with: Human
- Product overview
Human Apolipoprotein AI (Apo A1) comprises about 70% of the high-density lipoproteins (HDL) protein mass and Apo A1I another 15–20%. Apo A1, a 243-amino acid molecule that contains a series of highly homologous amphipathic alpha-helices, is a 28-kDa single polypeptide that lacks glycosylation or disulfide linkages. About 5–10% of human plasma Apo A1 exists in a lipoprotein-unassociated state. Apo A1 appears to have effects on the atherosclerosis inhibition, reverse cholesterol transport and anti-inflammation. Oxidation of specific amino acid residues in Apo A1 may contribute to atherogenesis by impairing cholesterol efflux from macrophages. A majority of HDL functionality is derived from the ability of Apo A1 to sequester phospholipid and cholesterol and interact with plasma enzymes and cellular receptors. During reverse cholesterol transport, HDL interacts with lecithin: cholesteryl acyltransferase (LCAT) and cellular receptors, including ATP-binding cassette transporter protein A1 (ABCA1) and the scavenger receptor class B type I in an ordered fashion that is reflected by HDL particle lipid composition. A high-affinity HDL receptor for Apo A1 is beta-chain of ATP synthase on the surface of hepatocytes. The plasma concentration of Apo A1 is one of the best indicators of susceptibility to cardiovascular disease.
Ab108804 Human Apo A1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of human Apo A1 in plasma and serum. This assay employs a quantitative competitive enzyme immunoassay technique that measures human Apo A1 in less than 3 hours. A polyclonal antibody specific for human ApoA-1 has been pre-coated onto a 96-well microplate with removable strips. Apo A1 in standards and samples is competed with a biotinylated Apo A1 sandwiched by the immobilized antibody and streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
- Tested applicationsELISA, Competitive ELISA more details
- PlatformMicroplate
Properties
- Storage instructionsStore at +4°C. Please refer to protocols.
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Components 1 x 96 tests Biotinylated Apo A-1 1 unit Chromogen Substrate 1 x 8ml Diluent Concentrate (10x) 1 x 30ml Human Apo A-1 Microplate 1 unit Human Apo A-1 Standard 1 unit Stop Solution 1 x 12ml Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 80µl Wash Buffer Concentrate (20x) 1 x 30ml - Research Areas
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Alternative names
- Apo AIApo-AIApo-AI
- ApoA IApoA-IApoA-IAPOA1APOA1/APOC3 fusion geneAPOA1_HUMANApolipoprotein A I precursorApolipoprotein A-IApolipoprotein A-I(1-242)Apolipoprotein A-I(1-242)Apolipoprotein A1Apolipoprotein A1Apolipoprotein AIApolipoprotein of high density lipoproteinApolipoproteinAIBrp14Ltw1Lvtw1MGC117399Sep1Sep2
see all
- Entrez Gene: 335 Human
- Omim: 107680 Human
- SwissProt: P02647 Human
Applications
Our Abpromise guarantee covers the use of ab108804 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ELISA | ELISA |
| Competitive ELISA | cELISA |
Apolipoprotein A1 Human ELISA Kit images
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- Apolipoprotein A1 Human ELISA Kit (ab108804)
Protocols
References for Apolipoprotein A1 Human ELISA Kit (ab108804)
ab108804 has not yet been referenced specifically in any publications.
