Anti-Apolipoprotein B antibody (HRP) (ab8948)

Overview

• Product nameAnti-Apolipoprotein B antibody (HRP)See all Apolipoprotein B primary antibodies ...
• Description
  Sheep polyclonal to Apolipoprotein B (HRP)
• ConjugationHRP
• SpecificityThe unconjugated immunoglobulin gives a single arc when tested by immunoelectrophoresis against Human plasma. Identity has been confirmed by double diffusion (Ouchterlony) against Human plasma and an anti-Humanapolipoprotein B of known specificity.
• Tested applicationsELISA more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Human Apolipoprotein B purified from plasma and shown to be homogeneous by SDS-PAGE.

• General notes

  
  

  Apolipoprotein B consists of a single polypeptide chain with a molecular weight of 549 kDa and is most lysynthesised in the liver. It is a major apolipoprotein of very low density, intermediate density and low density lipoproteins (LDL) as well as being a major component of lipoprotein (a). Apolipoprotein B is a ligand for the LDL receptor and elevated levels are associated with premature atherosclerosis. Normal plasma apolipoprotein B levels are around 800mg/L.3

Properties

• FormLiquid
• Storage instructionsStore at +4°C.
• Storage bufferpH: 7.20
  Preservative: 0.05% Proclin
  Constituents: 1% BSA, PBS
• Concentration information loading...
• PurityIgG fraction
• Purification notesAntiserum is prepared by immunisation of sheep with Apolipoprotein B and if necessary, adsorped. Following adsorption, an immunoglobulin fraction is produced by ion-exchange chromatography and zone electrophoresis is performed to ensure that only agamma-mobility component is present. The antibody is then conjugated with horseradish peroxidase activated by periodate. Preservatives are added and the product is then 0.2µm filtered.
• Primary antibody notes Apolipoprotein B consists of a single polypeptide chain with a molecular weight of 549 kDa and is most lysynthesised in the liver. It is a major apolipoprotein of very low density, intermediate density and low density lipoproteins (LDL) as well as being a major component of lipoprotein (a). Apolipoprotein B is a ligand for the LDL receptor and elevated levels are associated with premature atherosclerosis. Normal plasma apolipoprotein B levels are around 800mg/L.3
• Clonality Polyclonal
• IsotypeIgG
• Light chain typeunknown
• Research Areas
  • Metabolism
  • Types of disease
  • Heart disease

  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Lipid and lipoprotein metabolism
  • Lipid metabolism

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Lipid and lipoprotein metabolism
  • Cholesterol Metabolism

  • Cancer
  • Cancer Metabolism
  • Metabolic signaling pathway
  • Metabolism of lipids and lipoproteins

  • Signal Transduction
  • Metabolism
  • Lipid metabolism

  • Cardiovascular
  • Atherosclerosis
  • Thrombosis
  • Fibrinolysis

  • Cardiovascular
  • Lipids / Lipoproteins
  • Lipid Metabolism
  • Cholesterol Metabolism

  • Cardiovascular
  • Lipids / Lipoproteins
  • Lipoproteins/Apolipoproteins
  • Apolipoproteins

Applications

Target

• FunctionApolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
• Involvement in diseaseFamilial hypobetalipoproteinemia 1 (FHBL1) [MIM:107730]: A disorder of lipid metabolism characterized by less than 5th percentile age- and sex-specific levels of low density lipoproteins, and dietary fat malabsorption. Clinical presentation may vary from no symptoms to severe gastrointestinal and neurological dysfunction similar to abetalipoproteinemia. Note=The disease is caused by mutations affecting the gene represented in this entry.
  Familial ligand-defective apolipoprotein B-100 (FDB) [MIM:144010]: Dominantly inherited disorder of lipoprotein metabolism leading to hypercholesterolemia and increased proneness to coronary artery disease (CAD). The plasma cholesterol levels are dramatically elevated due to impaired clearance of LDL particles by defective APOB/E receptors. Note=The disease is caused by mutations affecting the gene represented in this entry.
  Note=Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia.
• Sequence similaritiesContains 1 vitellogenin domain.
• Post-translational
  modificationsPalmitoylated; structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle.
• Cellular localizationCytoplasm. Secreted.

Target information above from: UniProt accession P04114 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 338 Human
  • Omim: 107730 Human
  • SwissProt: P04114 Human
  • Unigene: 120759 Human

• Alternative names
  Apo B 100 antibodyApo B antibodyApo B-100 antibody

  Apo B-48 antibodyApoB 100 antibodyApoB 48 antibodyApoB antibodyAPOB protein antibodyAPOB_HUMAN antibodyApolipoprotein B 100 antibodyApolipoprotein B 48 antibodyApolipoprotein B antibodyApolipoprotein B-48 antibodyApolipoprotein B48 antibodyFLDB antibodyLDLCQ4 antibody

  see all

Protocols

• ab8948 ELISA recommendations