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Anti-Apolipoprotein B antibody [7B8] (ab39560)

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Overview

  • Product nameAnti-Apolipoprotein B antibody [7B8]See all Apolipoprotein B primary antibodies ...
  • Description
    Mouse monoclonal [7B8] to Apolipoprotein B
  • SpecificityAb39560 has specificity for Apolipoprotein B from serum/plasma. No crossreactivity is seen with Apolipoprotein A1.
  • Tested applicationsELISA, WB more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human LDL.

Applications

Our Abpromise guarantee covers the use of ab39560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ELISA ELISA: Use at an assay dependent dilution. Ab39560 reacts with Apolipoprotein B from human serum/plasma, when used in sandwich ELISA in combination with a polyclonal antibody against Apolipoprotein B, and also with isolated LDL directly coated onto the microtiter plate.
WB WB: Use at an assay dependent dilution. Predicted molecular weight: 260 kDa. Ab39560 binds to Apolipoprotein B in reduced as well as in non-reduced forms, using isolated LDL as antigen.

Target

  • FunctionApolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
  • Involvement in diseaseFamilial hypobetalipoproteinemia 1 (FHBL1) [MIM:107730]: A disorder of lipid metabolism characterized by less than 5th percentile age- and sex-specific levels of low density lipoproteins, and dietary fat malabsorption. Clinical presentation may vary from no symptoms to severe gastrointestinal and neurological dysfunction similar to abetalipoproteinemia. Note=The disease is caused by mutations affecting the gene represented in this entry.
    Familial ligand-defective apolipoprotein B-100 (FDB) [MIM:144010]: Dominantly inherited disorder of lipoprotein metabolism leading to hypercholesterolemia and increased proneness to coronary artery disease (CAD). The plasma cholesterol levels are dramatically elevated due to impaired clearance of LDL particles by defective APOB/E receptors. Note=The disease is caused by mutations affecting the gene represented in this entry.
    Note=Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia.
  • Sequence similaritiesContains 1 vitellogenin domain.
  • Post-translational
    modifications    Palmitoylated; structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle.
  • Cellular localizationCytoplasm. Secreted.

    • Target information above from: UniProt accession P04114 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        Apo B 100 antibodyApo B antibodyApo B-100 antibody
        Apo B-48 antibodyApoB 100 antibodyApoB 48 antibodyApoB antibodyAPOB protein antibodyAPOB_HUMAN antibodyApolipoprotein B 100 antibodyApolipoprotein B 48 antibodyApolipoprotein B antibodyApolipoprotein B-48 antibodyApolipoprotein B48 antibodyFLDB antibodyLDLCQ4 antibody
      see all

    References for Anti-Apolipoprotein B antibody [7B8] (ab39560)

    ab39560 has not yet been referenced specifically in any publications.

    Publishing research using ab39560 ? Please let us know so that we can cite the reference in this datasheet

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    Submit an Abreview for ab39560 Submit a Question for ab39560

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    Hi,



    I emailed about this antibody last week but I have a quicker questions which I'm hoping you can answer.



    I’m wondering what species of apoB your antibody (ab39560) reacts with. I understand the immuno...

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    Thank you for your patience.

    I now heard back form the lab and they confirmed that was never experimentally tested if ab39560 also binds to apoB48 alone.

    As stated on the datasheet ab39560 reacts with Apolipoprotein B from human serum/...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"