Apolipoprotein CI Human ELISA Kit (ab108808)
- Product nameApolipoprotein CI Human ELISA Kit
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity= 0.06 µg/ml
- Range0.063 µg/ml - 4 µg/ml
- Species reactivityReacts with: Human
Apolipoprotein C-I (ApoC-I) is a 6.6 kDa apolipoprotein that is expressed primarily in the liver and activated when monocytes differentiate into macrophases. After being synthesized as a precursor with a length of 83 amino acids, apoC-I is processed to a single chain mature protein of 57 amino acids. It circulates in plasma and is a component of VLDL, IDL, and HDL. ApoC-I plays important modulatory roles in lipoprotein metabolism. It is an inhibitor of lipoprotein binding to the LDL receptor, LDL receptor-related protein, and VLDL receptor. It is the major plasma inhibitor of cholesteryl ester transfer protein and appears to interfere directly with fatty acid uptake. ApoC-I causes hypertriglyceridemia by inhibition of the lipoprotein lipase-dependent triglyceride-hydrolysis pathway. On the other hand, apoC-I is an activator of lecithin cholesterol acyl transferase that esterifies cholesterol and produces the formation of the mature HDL. It is also a physiological protector against infection by enhancing the early inflammatory response to lipopolysaccharide.
ab108808 Apolipoprotein CI Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of human Apo C-I in plasma, serum and cell culture samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures human Apo C-I in less than 5 hours. A polyclonal antibody specific for human Apo C-I has been pre-coated onto a 96-well microplate with removable strips. Apo C-I in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for Apo C-I, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
- Tested applicationsELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests Biotinylated Apo CI Antibody (50x) 1 x 140µl Chromogen Substrate 1 x 8ml Diluent Concentrate (10x) 1 x 30ml Human Apo CI Microplate 1 unit Human Apo CI Standard 2 units Stop Solution 1 x 12ml Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 80µl Wash Buffer Concentrate (20x) 2 x 30ml
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
- APO C1Apo CIApo-CIB
- Apo-CIB'APOC 1ApoC IApoC-IBApoC-IB'APOC1APOC1BApolipoprotein C IApolipoprotein C-IApolipoprotein CIApolipoproteinC IApolipoproteinCITruncated apolipoprotein C-I
- SwissProt: P02654 Human
Our Abpromise guarantee covers the use of ab108808 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Apolipoprotein CI Human ELISA Kit (ab108808)
ab108808 has not yet been referenced specifically in any publications.