Overview
- Product nameAnti-Apolipoprotein CI antibodySee all Apolipoprotein CI primary antibodies ...
- DescriptionRabbit polyclonal to Apolipoprotein CI
- SpecificityAb20793 specifically binds to human Apo CI. No cross-reactions with Human Apo AI, Apo AII, Apo B-100, Apo CII, Apo CIII, or Apo E.
- Tested applicationsICC/IF, Indirect ELISA, WB, ELISA more details
- Species reactivityReacts with: Human
- Immunogen
Full length protein (Human Apolipoprotein-CI).
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 75mM PBS, 75mM Sodium chloride, 1mM EDTA. pH 7.2 -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab20793 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ICC/IF | ICC/IF: Use a concentration of 5 mg/ml. |
| Indirect ELISA | I-ELISA: Use at an assay dependent concentration. |
| WB | WB: 1/5000 - 1/20000. Predicted molecular weight: 9 kDa. |
| ELISA | ELISA: 1/5000 - 1/20000. Dilution optimised using Chromogenic detection. |
Target
- RelevanceThe protein encoded by this gene is a member of the apolipoprotein C1 family. This gene is expressed primarily in the liver, and it is activated when monocytes differentiate into macrophages. A pseudogene of this gene is located 4 kb downstream in the same orientation, on the same chromosome. This gene is mapped to chromosome 19, where it resides within a apolipoprotein gene cluster. Alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. Apolipoprotein C-I appears to modulate the interaction of APOE with beta migrating VLDL and inhibit binding of beta VLDL to the LDL receptor related protein. At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL and VLDL particles in human serum are often used to estimate an individuals' relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins (especially A1 and B) have been suggested to be more accurate estimators of coronary heart disease than measurements of lipoprotein particles (especially HDL and LDL).
- Cellular localizationSecreted
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Database links
- Entrez Gene: 341 Human
- Entrez Gene: 341 Human
- Omim: 107710 Human
- SwissProt: P02654 Human
- SwissProt: P02654 Human
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Alternative names
- APO C1 antibodyApo CI antibodyApo-CIB antibody
- Apo-CIB' antibodyAPOC 1 antibodyApoC I antibodyApoC-IB antibodyApoC-IB' antibodyAPOC1 antibodyAPOC1B antibodyApolipoprotein C I antibodyApolipoprotein C-I antibodyApolipoprotein CI antibodyApolipoproteinC I antibodyApolipoproteinCI antibodyTruncated apolipoprotein C-I antibody
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Anti-Apolipoprotein CI antibody images
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Immunocytochemistry/ Immunofluorescence-Apolipoprotein CI antibody(ab20793)ICC/IF image of ab20793 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab20793, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Apolipoprotein CI antibody (ab20793)
ab20793 has not yet been referenced specifically in any publications.
