Anti-Apolipoprotein CI antibody (ab7617)

Overview

  • Product nameAnti-Apolipoprotein CI antibodySee all Apolipoprotein CI primary antibodies ...
  • Description
    Goat polyclonal to Apolipoprotein CI
  • SpecificityTypically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA. This antibody reacts with human apoLipoprotein C-I and has negligible cross-reactivity with Type A-I, A-II, B, C-II, C-III, E and J apoLipoproteins.
  • Tested applicationsDot Blot, ELISA, WB more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length synthetic apoLipoprotein Type C-I (purified).

  • General notesThis antibody has been used to determine that atherosclerotic lesions in the human aorta contain considerable amounts of lipoproteins. These lipoproteins were observed to be complexed with components of the extracellular matrix (especially LDL and proteoglycans). The role of these matrix-lipoprotein complexes is not entirely clear, however, animal models of atherosclerosis have shown that increased cellular proliferation and increased production of extracellular matrix components occur following injury to the intimal layer of the aorta.

Properties

Applications

Our Abpromise guarantee covers the use of ab7617 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Dot Blot 1/5000 - 1/10000.
ELISA 1/4000 - 1/8000.
WB 1/1000.

Target

  • RelevanceThe protein encoded by this gene is a member of the apolipoprotein C1 family. This gene is expressed primarily in the liver, and it is activated when monocytes differentiate into macrophages. A pseudogene of this gene is located 4 kb downstream in the same orientation, on the same chromosome. This gene is mapped to chromosome 19, where it resides within a apolipoprotein gene cluster. Alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. Apolipoprotein C-I appears to modulate the interaction of APOE with beta migrating VLDL and inhibit binding of beta VLDL to the LDL receptor related protein. At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL and VLDL particles in human serum are often used to estimate an individuals' relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins (especially A1 and B) have been suggested to be more accurate estimators of coronary heart disease than measurements of lipoprotein particles (especially HDL and LDL).
  • Cellular localizationSecreted
  • Database links
  • Alternative names
    • APO C1 antibody
    • Apo CI antibody
    • Apo-CIB antibody
    • Apo-CIB' antibody
    • APOC 1 antibody
    • ApoC I antibody
    • ApoC-IB antibody
    • ApoC-IB' antibody
    • APOC1 antibody
    • APOC1B antibody
    • Apolipoprotein C I antibody
    • Apolipoprotein C-I antibody
    • Apolipoprotein CI antibody
    • ApolipoproteinC I antibody
    • ApolipoproteinCI antibody
    • Truncated apolipoprotein C-I antibody
    see all

References for Anti-Apolipoprotein CI antibody (ab7617)

ab7617 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your phone call. I enquired with the originator of this antibody and it turns out that ab7617 has not yet been tested for application in IHC. There was an error on the datasheet which I have updated and I apologize for any confusion. This...

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Thank you for your enquiry. The Apo C1 is purified from Human plasma by density gradient ultracentrifugation and HPLC. The protocol of the purification is proprietary. A general WB protocol is followed. This product has been assayed against 1...

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Thank you for your enquiry. The antibody was tested in WB (6.6kDa) and Elisa. The sample tested against was purified Human apoC1 protein. I hope this information helps, please do not hesitate to contact us if you need any more advice or informat...

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I'm sorry to hear you are having problems with ab7617 in WB. I would like to recommend incubating the primary antibody overnight at 4C to promote binding of the antibody to the protein and using ECL+ as a detection kit as this is more sensitive than E...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"