Apolipoprotein CIII Monkey ELISA Kit (ab108812)
- Product nameApolipoprotein CIII Monkey ELISA KitSee all Apolipoprotein CIII kits ...
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity= 0.07 µg/ml
- Range0.078 µg/ml - 5 µg/ml
- Species reactivityReacts with: Monkey
Apolipoprotein C-III (ApoC-III) is a surface component of chylomicrons, very low density lipoproteins, and high density lipoproteins. It consists of 79 amino acids with a molecular mass of 8.8 kDa . ApoC-III is synthesized mainly in the liver and to a lesser degree in the intestine. It plays a key role in triglyceride-rich lipoprotein metabolism. It is an inhibitor of lipoprotein lipase and hepatic lipase, and interferes with binding of lipoproteins to cell surface heparan sulfate proteoglycans and receptors. Overexpression of the human ApoC-III gene causes hypertriglyceridemia in transgenic mice. Deficiency of ApoC-III prevents hyperlipidemia induced by ApoE overexpression. As its deficiency results in diet-induced obesity and aggravated insulin resistance in mice, ApoC3 is a potential target for treatment of obesity and insulin resistance.
ab108812 Monkey ApoC-III ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of monkey ApoC-III in plasma, serum and cell culture samples. This assay employs a quantitative competitive enzyme immunoassay technique that measures monkey ApoC-III in less than 5 hours. A mAb capture antibody has been coated onto a 96-well microplate with removable strips. Monoclonal antibody against monkey ApoC-III binds to the captured antibody. ApoC-III in standards and samples is competed by a biotinylated ApoC-III sandwiched by the immobilized antibody and streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
- Tested applicationsELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests Biotinylated Apo C-III 1 unit Chromogen Substrate 1 x 8ml Diluent Concentrate (10x) 1 x 30ml mAb Capture Microplate: 1 unit Monkey Apo C-III Standard 1 unit Monoclonal Antibody against Monkey Apo C-III 1 x 180µl Stop Solution 1 x 12ml Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 80µl Wash Buffer Concentrate (20x) 2 x 30ml
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
modificationsO-linked glycan consists of Gal-GalNAc disaccharide, further modified with up to 3 sialic acid residues. O-glycosylated on Thr-94 with a core 1 or possibly core 8 glycan.
- APOC3APO C3Apo CIII
- Apo-CIIIAPOC 3ApoC IIIApoC-IIIAPOC3APOC3APOC3_HUMANApoCIIIApolipoprotein C IIIApolipoprotein C-IIIApolipoprotein C3ApolipoproteinCIIIMGC150353
Our Abpromise guarantee covers the use of ab108812 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Apolipoprotein CIII Monkey ELISA Kit images
This curve is provided for illustration only. A standard curve should be generated each time the assay is performed.
References for Apolipoprotein CIII Monkey ELISA Kit (ab108812)
ab108812 has not yet been referenced specifically in any publications.