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Overview

  • Product nameApolipoprotein CIII Monkey ELISA KitSee all Apolipoprotein CIII kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    = 0.07 µg/ml
  • Range
    0.078 µg/ml - 5 µg/ml
  • Recovery

    97 %

  • Species reactivity
    Reacts with: Monkey
  • Notes

    Apolipoprotein C-III (ApoC-III) is a surface component of chylomicrons, very low density lipoproteins, and high density lipoproteins. It consists of 79 amino acids with a molecular mass of 8.8 kDa . ApoC-III is synthesized mainly in the liver and to a lesser degree in the intestine. It plays a key role in triglyceride-rich lipoprotein metabolism. It is an inhibitor of lipoprotein lipase and hepatic lipase, and interferes with binding of lipoproteins to cell surface heparan sulfate proteoglycans and receptors. Overexpression of the human ApoC-III gene causes hypertriglyceridemia in transgenic mice. Deficiency of ApoC-III prevents hyperlipidemia induced by ApoE overexpression. As its deficiency results in diet-induced obesity and aggravated insulin resistance in mice, ApoC3 is a potential target for treatment of obesity and insulin resistance.

    ab108812 Monkey ApoC-III ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of monkey ApoC-III in plasma, serum and cell culture samples. This assay employs a quantitative competitive enzyme immunoassay technique that measures monkey ApoC-III in less than 5 hours. A mAb capture antibody has been coated onto a 96-well microplate with removable strips. Monoclonal antibody against monkey ApoC-III binds to the captured antibody. ApoC-III in standards and samples is competed by a biotinylated ApoC-III sandwiched by the immobilized antibody and streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

  • Tested applicationsELISA more details

Properties

  • FunctionInhibits lipoprotein lipase and hepatic lipase and decreases the uptake of lymph chylomicrons by hepatic cells. This suggests that it delays the catabolism of triglyceride-rich particles.
  • Tissue specificityConstitutes 50% of the protein fraction of VLDL and 2% of that of HDL. Synthesized predominantly in liver and to a lesser degree in intestine.
  • Involvement in diseaseDefects in APOC3 are the cause of hyperalphalipoproteinemia type 2 (HALP2) [MIM:614028]. HALP2 is a condition characterized by high levels of high density lipoprotein (HDL) and increased HDL cholesterol levels.
  • Sequence similaritiesBelongs to the apolipoprotein C3 family.
  • Post-translational
    modifications    O-linked glycan consists of Gal-GalNAc disaccharide, further modified with up to 3 sialic acid residues. O-glycosylated on Thr-94 with a core 1 or possibly core 8 glycan.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P02656 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        APOC3APO C3Apo CIII
        Apo-CIIIAPOC 3ApoC IIIApoC-IIIAPOC3APOC3APOC3_HUMANApoCIIIApolipoprotein C IIIApolipoprotein C-IIIApolipoprotein C3ApolipoproteinCIIIMGC150353
      see all

    Applications

    Our Abpromise guarantee covers the use of ab108812 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    ELISA ELISA

    Apolipoprotein CIII Monkey ELISA Kit images

    • ELISA - Apolipoprotein CIII Monkey ELISA Kit (ab108812)
      ELISA - Apolipoprotein CIII Monkey ELISA Kit (ab108812)
      This curve is provided for illustration only. A standard curve should be generated each time the assay is performed.

    Protocols

    References for Apolipoprotein CIII Monkey ELISA Kit (ab108812)

    ab108812 has not yet been referenced specifically in any publications.

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