Apolipoprotein CIII Rat/Mouse ELISA Kit (ab108811)
- Product nameApolipoprotein CIII Rat/Mouse ELISA KitSee all Apolipoprotein CIII kits ...
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma, Cell Lysate
- Assay typeSandwich
- Sensitivity= 0.5 µg/ml
- Range0.5 µg/ml - 8 µg/ml
- Assay time5h 00m
- Species reactivityReacts with: Mouse, Rat
- Product overview
ab108811 Apolipoprotiein CIII Rat/Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of rat/mouse Apo C-III in plasma, serum and cell culture samples. This assay employs a quantitative competitive enzyme immunoassay technique that measures rat/mouse Apo C-III in less than 5 hours. A capture antibody has been coated onto a 96-well microplate with removable strips. A specific antibody against Rat Apo C-III binds to the captured antibody. Apo C-III in standards and samples is competed by a biotinylated Apo C-III sandwiched by the immobilized antibody and streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
Apolipoprotein CIII (apoC-III) is a surface component of chylomicrons, very low density lipoproteins, and high density lipoproteins. It consists of 79 amino acids with a molecular mass of 8.8 kDa. ApoC-III is synthesized mainly in the liver and to a lesser degree in the intestine. It plays a key role in triglyceride-rich lipoprotein metabolism. It is an inhibitor of lipoprotein lipase and hepatic lipase, and interferes with binding of lipoproteins to cell surface heparan sulfate proteoglycans and receptors. Overexpression of the human ApoC-III gene causes hypertriglyceridemia in transgenic mice. Deficiency of ApoC-III prevents hyperlipidemia induced by ApoE overexpression. As its deficiency results in diet-induced obesity and aggravated insulin resistance in mice, ApoC3 is a potential target for treatment of obesity and insulin resistance.
- Tested applicationsELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests Antibody Capture Microplate 1 unit Biotinylated Apo C-III (2x) 1 unit Chromogen Substrate 1 x 8ml Diluent Concentrate (10x) 1 x 30ml Rat Apo C-III Standard 1 unit Specific Antibody against Rat Apo C-III (10x) 1 x 520µl Stop Solution 1 x 12ml Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 80µl Wash Buffer Concentrate (20x) 2 x 30ml
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
modificationsO-linked glycan consists of Gal-GalNAc disaccharide, further modified with up to 3 sialic acid residues. O-glycosylated on Thr-94 with a core 1 or possibly core 8 glycan.
- APOC3APO C3Apo CIII
- Apo-CIIIAPOC 3ApoC IIIApoC-IIIAPOC3APOC3APOC3_HUMANApoCIIIApolipoprotein C IIIApolipoprotein C-IIIApolipoprotein C3ApolipoproteinCIIIMGC150353
Our Abpromise guarantee covers the use of ab108811 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Apolipoprotein CIII Rat/Mouse ELISA Kit images
This curve is provided for illustration only. A standard curve should be generated each time the assay is performed.
References for Apolipoprotein CIII Rat/Mouse ELISA Kit (ab108811)
ab108811 has not yet been referenced specifically in any publications.