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Apolipoprotein CIII Rat/Mouse ELISA Kit (ab108811)

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Overview

  • Product nameApolipoprotein CIII Rat/Mouse ELISA KitSee all Apolipoprotein CIII kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma, Cell Lysate
  • Assay typeSandwich
  • Sensitivity
    = 0.5 µg/ml
  • Range
    0.5 µg/ml - 8 µg/ml
  • Recovery

    98 %

  • Assay time
    5h 00m
  • Species reactivity
    Reacts with: Mouse, Rat
  • Product overview

    ab108811 Apolipoprotiein CIII Rat/Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of rat/mouse Apo C-III in plasma, serum and cell culture samples. This assay employs a quantitative competitive enzyme immunoassay technique that measures rat/mouse Apo C-III in less than 5 hours. A capture antibody has been coated onto a 96-well microplate with removable strips. A specific antibody against Rat Apo C-III binds to the captured antibody. Apo C-III in standards and samples is competed by a biotinylated Apo C-III sandwiched by the immobilized antibody and streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

  • Notes

    Apolipoprotein CIII (apoC-III) is a surface component of chylomicrons, very low density lipoproteins, and high density lipoproteins. It consists of 79 amino acids with a molecular mass of 8.8 kDa. ApoC-III is synthesized mainly in the liver and to a lesser degree in the intestine. It plays a key role in triglyceride-rich lipoprotein metabolism. It is an inhibitor of lipoprotein lipase and hepatic lipase, and interferes with binding of lipoproteins to cell surface heparan sulfate proteoglycans and receptors. Overexpression of the human ApoC-III gene causes hypertriglyceridemia in transgenic mice. Deficiency of ApoC-III prevents hyperlipidemia induced by ApoE overexpression. As its deficiency results in diet-induced obesity and aggravated insulin resistance in mice, ApoC3 is a potential target for treatment of obesity and insulin resistance.

     

  • Tested applicationsELISA more details

Properties

  • FunctionInhibits lipoprotein lipase and hepatic lipase and decreases the uptake of lymph chylomicrons by hepatic cells. This suggests that it delays the catabolism of triglyceride-rich particles.
  • Tissue specificityConstitutes 50% of the protein fraction of VLDL and 2% of that of HDL. Synthesized predominantly in liver and to a lesser degree in intestine.
  • Involvement in diseaseDefects in APOC3 are the cause of hyperalphalipoproteinemia type 2 (HALP2) [MIM:614028]. HALP2 is a condition characterized by high levels of high density lipoprotein (HDL) and increased HDL cholesterol levels.
  • Sequence similaritiesBelongs to the apolipoprotein C3 family.
  • Post-translational
    modifications    O-linked glycan consists of Gal-GalNAc disaccharide, further modified with up to 3 sialic acid residues. O-glycosylated on Thr-94 with a core 1 or possibly core 8 glycan.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P02656 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        APOC3APO C3Apo CIII
        Apo-CIIIAPOC 3ApoC IIIApoC-IIIAPOC3APOC3APOC3_HUMANApoCIIIApolipoprotein C IIIApolipoprotein C-IIIApolipoprotein C3ApolipoproteinCIIIMGC150353
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab108811 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      Apolipoprotein CIII Rat/Mouse ELISA Kit images

      • ELISA - Apolipoprotein CIII Rat/Mouse ELISA Kit (ab108811)
        ELISA - Apolipoprotein CIII Rat/Mouse ELISA Kit (ab108811)
        This curve is provided for illustration only. A standard curve should be generated each time the assay is performed.

      Protocols

      References for Apolipoprotein CIII Rat/Mouse ELISA Kit (ab108811)

      ab108811 has not yet been referenced specifically in any publications.

      Publishing research using ab108811 ? Please let us know so that we can cite the reference in this datasheet

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      Submit an Abreview for ab108811 Submit a Question for ab108811

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      10
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      To whom it may concern at abcam.
      Could you please tell me if you have detected ApoCIII in rat serum using this ELISA?

      Thank you,
      Sincerely



      Please find the comments from the lab below.

      "Yes, this kit is tested and guaranteed for rat serum. I would say that the average value of ApoCIII in our rat sample is 85 ug/ml. However, it can be lower or much higher depending on ...

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      Inquiry: I would like to measure apolipoprotein CIII in serum from rat and mouse. I have previously tested a mouse/rat ELISA from Abnova that looks very similar to what I can read about your ELISA. The Abnova ELISA did not work I have tested three differe...

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      Thank you for your enquiry.

      Reviewing the details, I can confirm that ab108811Apolipoprotein CIII Rat/Mouse ELISA Kit and the AbNova kit you describe works on a competitive ELISA protocol. Therefore, the absorbence for blank is expected to be v...

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      Inquiry: Hello, Could you provide information on the Diluent buffer for AB108811? The reason for the question is that our plasma sample has been separated (and diluted) by chromatography and we do not want to dilute 1:80 in diluent. Will this affect the a...

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      Thank you for your inquiry.

      The components of the diluent buffer are proprietary.

      You can always test the dilution factor. Our samples work well at 80x, however your samples could work well with a different dilution factor other than 1...

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      We would like to purchase this with the promotional discount towards next purchase.

      DISCOUNT CODE: ************
      Expiration date: *********

      I am very pleased to hear you would like to accept our offer and test ab108811 in golden syrian hamster plasma. This code will give you:$470.00 VALUE OFF YOUR NEXT ORDER before the exp...

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      : Hello, Will this ELISA work for measuring ApoCIII in golden syrian hamster plasma? Thank you,

      Thank you very much for your interest in ab108811.

      To our knowledge, ab108811 has not been tested in golden syrian hamster plasma. Therefore, I can offer a discount off a future purchase if you buy ab108811 now, test it in golden syrian hamster...

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      I want to know if you have mouse APOA1, APOB and APOCIII ELISAs? If you have, please give me a quotation of two for each of them.

      Thank you for contacting us. We currently only have a mouse ELISA kit for Apolipoprotein CIII: ab108811 However, we do have antibodies to each of your targets of interest that can be used in ELISA on mouse samples: Apolipoprotein A I: ab7614 or ab20453 Ap...

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      Dear Abcam Two short questions; Is the antibody in your kit  Apolipoprotein CIII Rat/Mouse ELISA Kit (ab108811)  identical to your anti ApoC3 antibody (ab55984)? Is it possible to by some purified/recombinant  rat ApoC3 from you or do you have a...

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      Thank you for contacting us. Thank you for contacting us. The Anti-Apolipoprotein CIII antibody ab55984 is not exatcly the same as that supplied with the Apolipoprotein CIII Rat/Mouse ELISA Kit (ab108811). The kit uses an antibody directed towards Rat...

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      Questions: 1. Would the kit be suitable for mouse plasma? 2. What is the level of ApoCIII in plasma? How dense is it- maybe other non-ApoCIII proteins will be in the way of detection? 3. Another option is to use VLDL or chlyomicron fraction? Is there a...

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      Answers: 1. Yes 2. Apo C-III level in plasma: 60-150 ul/ml 3. We have never tested VLDL or chylomicron fraction with this kit. We suggest 1:80 as a starting dilution of mouse plasma so for example you can use 4 ul of your original mouse plasma sample ...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"