Anti-Apolipoprotein CIII antibody (ab84290)

Overview

• Product nameAnti-Apolipoprotein CIII antibodySee all Apolipoprotein CIII primary antibodies ...
• Description
  Rabbit polyclonal to Apolipoprotein CIII
• Tested applicationsIHC-P, ICC/IF more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 50 to the C-terminus of Human Apolipoprotein CIII .

  Read Abcam's proprietary immunogen policy

• Positive controlThis antibody gave a positive signal in a human liver carcinoma tissue section.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Lipid and lipoprotein metabolism
  • Lipid metabolism

  • Metabolism
  • Types of disease
  • Cancer

  • Cancer
  • Cancer Metabolism
  • Metabolic signaling pathway
  • Metabolism of lipids and lipoproteins

  • Signal Transduction
  • Metabolism
  • Lipid metabolism

  • Cardiovascular
  • Lipids / Lipoproteins
  • Lipoproteins/Apolipoproteins
  • Apolipoproteins

Applications

Target

• FunctionInhibits lipoprotein lipase and hepatic lipase and decreases the uptake of lymph chylomicrons by hepatic cells. This suggests that it delays the catabolism of triglyceride-rich particles.
• Tissue specificityConstitutes 50% of the protein fraction of VLDL and 2% of that of HDL. Synthesized predominantly in liver and to a lesser degree in intestine.
• Involvement in diseaseDefects in APOC3 may be a cause of hyperalphalipoproteinemia (HYPALIP) [MIM:143470]. Affected individuals show high levels of alpha-lipoprotein (high density lipoprotein/HDL).
• Sequence similaritiesBelongs to the apolipoprotein C3 family.
• Post-translational
  modificationsO-linked glycan consists of Gal-GalNAc disaccharide, further modified with up to 3 sialic acid residues. O-glycosylated on Thr-94 with a core 1 or possibly core 8 glycan.
• Cellular localizationSecreted.

Target information above from: UniProt accession P02656 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 345 Human
  • Entrez Gene: 345 Human
  • Omim: 107720 Human
  • SwissProt: P02656 Human
  • SwissProt: P02656 Human
  • Unigene: 73849 Human

• Alternative names
  APOC3 antibodyAPO C3 antibodyApo CIII antibody

  Apo-CIII antibodyAPOC 3 antibodyApoC III antibodyApoC-III antibodyAPOC3 antibodyAPOC3_HUMAN antibodyApoCIII antibodyApolipoprotein C III antibodyApolipoprotein C-III antibodyApolipoprotein C3 antibodyApolipoproteinCIII antibodyMGC150353 antibody

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Anti-Apolipoprotein CIII antibody images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Apolipoprotein CIII antibody (ab84290)
  IHC image of Apolipoprotein CIII staining in human liver carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab84290, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
• 

  Immunocytochemistry/ Immunofluorescence - Apolipoprotein CIII antibody (ab84290)

  ICC/IF image of ab84290 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab84290 at 1µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.