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Apolipoprotein CIII protein (Human) (ab77944)

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Overview

  • Product nameApolipoprotein CIII protein (Human)See all Apolipoprotein CIII proteins and peptides ...
  • Protein descriptionFull length native Apolipoprotein CIII (mature form) purified from human plasma, VLDL. Swiss ID = P02656.
  • Expression hostNative

    • Properties

  • Purity> 95 % by SDS-PAGE
  • FormLyophilised:Reconstitute with 59.9ul distilled water.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 10mM Ammonium bicarbonate, pH 7.4
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab77944 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB WB: Use at an assay dependent dilution. Ab77944 can be used as a WB positive control in conjunction with ab21032.
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.

    Protein info

    • Alternative names
        APOC3APO C3Apo CIII
        Apo-CIIIAPOC 3ApoC IIIApoC-IIIAPOC3APOC3_HUMANApoCIIIApolipoprotein C IIIApolipoprotein C-IIIApolipoprotein C3ApolipoproteinCIIIMGC150353
      see all
  • FunctionInhibits lipoprotein lipase and hepatic lipase and decreases the uptake of lymph chylomicrons by hepatic cells. This suggests that it delays the catabolism of triglyceride-rich particles.
  • Tissue specificityConstitutes 50% of the protein fraction of VLDL and 2% of that of HDL. Synthesized predominantly in liver and to a lesser degree in intestine.
  • Involvement in diseaseDefects in APOC3 may be a cause of hyperalphalipoproteinemia (HYPALIP) [MIM:143470]. Affected individuals show high levels of alpha-lipoprotein (high density lipoprotein/HDL).
  • Sequence similaritiesBelongs to the apolipoprotein C3 family.
  • Post-translational
    modifications    O-linked glycan consists of Gal-GalNAc disaccharide, further modified with up to 3 sialic acid residues. O-glycosylated on Thr-94 with a core 1 or possibly core 8 glycan.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P02656 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    Apolipoprotein CIII protein (Human) images

    • Western blot - Apolipoprotein CIII antibody (ab21032)
      Western blot - Apolipoprotein CIII antibody (ab21032)
      All lanes : Anti-Apolipoprotein CIII antibody (ab21032) at 1 µg/ml

      Lane 1 : Apolipoprotein CIII protein (Human) (ab77944) at 0.1 µg
      Lane 2 : Apolipoprotein CIII protein (Human) (ab77944) at 0.01 µg

      Secondary
      Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Exposure time : 1 minute

    References for Apolipoprotein CIII protein (Human) (ab77944)

    ab77944 has not yet been referenced specifically in any publications.

    Publishing research using ab77944 ? Please let us know so that we can cite the reference in this datasheet

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