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Anti-Apolipoprotein D antibody (ab113634)

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Overview

  • Product nameAnti-Apolipoprotein D antibodySee all Apolipoprotein D primary antibodies ...
  • Description
    Sheep polyclonal to Apolipoprotein D
  • Tested applicationsWB, ELISA more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein produced in E. coli: FHLGKCPNPP VQENFDVNKY PGRWYEIEKI PTTFENGRCI QANYSLMENG KIKVLNQELR ADGTVNQIEG EATPVNLTEP AKLEVKFSWF MPSAPYHILA TDYENYALVY SCTSISQSFH VDFAWILARN VALPPETVDS LKNILTSNNI DVKKMTVTDQ VNCPKLSAHH HHHH, corresponding to amino acids 23-189 of Human Apolipoprotein D. Contains a C terminal His-tag of 7 extra AA. The amino acid sequence corresponds to the UniProt P05090. The following modifications were made: Four amino acid exchanges were introduced at the surface of ApoD (Trp99His, Cys116Ser, Ile118Ser, Leu120Ser) to enhance the solubility of the recombinant protein and another three (Leu23Pro, Pro133Val, Asn134Ala) to facilitate its genetic manipulation.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notesReconstituted antibody can be stored at 4°C for a limited period of time; it does not show decline in activity after one week at 4°C.

Properties

Applications

Our Abpromise guarantee covers the use of ab113634 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use at an assay dependent dilution. Predicted molecular weight: 21 kDa.
ELISA ELISA: Use at an assay dependent dilution.

Target

  • FunctionAPOD occurs in the macromolecular complex with lecithin-cholesterol acyltransferase. It is probably involved in the transport and binding of bilin. Appears to be able to transport a variety of ligands in a number of different contexts.
  • Tissue specificityExpressed in liver, intestine, pancreas, kidney, placenta, adrenal, spleen, fetal brain tissue and tears.
  • Sequence similaritiesBelongs to the calycin superfamily. Lipocalin family.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P05090 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        APO D antibodyApo-D antibodyApoD antibody
        APOD protein antibodyAPOD_HUMAN antibodyApolipoprotein D antibodyApolipoproteinD antibody
      see all

    References for Anti-Apolipoprotein D antibody (ab113634)

    ab113634 has not yet been referenced specifically in any publications.

    Publishing research using ab113634 ? Please let us know so that we can cite the reference in this datasheet

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    Hi there - we are using ab113634 for human plasma apoD and would like to know if you have any evidence (maybe publications?) that the ab can cross react with mouse apoD (not mentioned in product overview but I would like to check)?
    Best regards

    Thank you for your enquiry regarding ab113634.

    I have done a blast search for you using the immunogen sequnce but it seems that there is only 75% homology between the human and the mouse Apolipoprotein D sequence. This means that the it is unlik...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"