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Overview

  • Product nameApolipoprotein E Human ELISA KitSee all Apolipoprotein E kits ...
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma, Cell culture extracts
  • Assay typeSandwich
  • Sensitivity
    = 0.03 µg/ml
  • Range
    0.031 µg/ml - 2 µg/ml
  • Recovery

    98 %

  • Assay time
    4h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Apolipoprotein E (apoE) is a 34 kDa polymorphic protein with 299 amino acids and occurs in all lipoprotein fractions in plasma. It is synthesized primarily by the liver and is a main apoprotein of the chylomicron. ApoE is essential for the normal catabolism of triglyceride-rich lipoprotein constituents and cardiovascular diseases. ApoE is also critical in several other important biological processes, including Alzheimer's disease, cognitive function, immunoregulation, cell signaling, and infectious diseases. There are three common isoforms of the protein: apoE3 is normal; while apoE2 and apoE4 are dysfunctional. ApoE deficiency causes type III hyperlipoproteinemia and premature atherosclerosis. ApoE is a major genetic risk factor for late-onset familial Alzheimer's disease and for cognitive deficits associated with aging. ApoE4 enhances HIV-1 cell entry in vitro and the ApoE epsilon4/epsilon4 genotype accelerates HIV disease progression.

    Ab108813 Human Apo E ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of human Apo E in plasma, serum, urine, and cell culture samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures Apo E in less than 4 hours. A polyclonal antibody specific for human Apo E has been pre-coated onto a 96-well microplate with removable strips. Apo E in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for Apo E, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

  • Tested applicationsSandwich ELISA more details
  • PlatformMicroplate

Properties

  • Alternative names
      AD2AD2Apo-E
      ApoEAPOEAPOEAApolipoprotein E3 apolipoprotein E3ApolipoproteinEApoprotein LDLCQ5LPG
    see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab108813 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Sandwich ELISA sELISA

      Apolipoprotein E Human ELISA Kit images

      • ELISA - Apolipoprotein E Human ELISA Kit (ab108813)
        ELISA - Apolipoprotein E Human ELISA Kit (ab108813)

      Protocols

      References for Apolipoprotein E Human ELISA Kit (ab108813)

      ab108813 has not yet been referenced specifically in any publications.

      Publishing research using ab108813 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab108813 Submit a Question for ab108813

      Displaying 1 - 7 of 7 results for Abreviews and Q&A

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      Votes: Highest First

      I need to tell you that your lysis buffer does not work with my cells at all. There is no protein detected compared to another lysis buffer we use. It would be great if you find out for me what the problem is.

      I am referring to ab108813 (Apolop...

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      Thank you for the clarification. I am not sure why the lysis buffer is failing for you, if you mean the one listed in the protocol:

      Cell Lysate: Rinse cells with cold PBS and then scrape the cells
      into a tube with 5 ml cold PBS with 0.5 M ...

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      I have a question about Protease inhibitor cocktail you mentioned in the Cell Lysate Preparation. What is the concentration? I assumed that it is 1:100 as recommended in my protease inhibitor cocktail data sheet.

      Yes, the cocktail that is used by the laboratory that validates this kit is likely to be equivalent to what you have. I recommend using the inhibitor cocktail you have as recommended on the datasheet that comes with it.

      I have a question about cold PBS with 0.5 M EDTA used to scrape cells (p. 7 of the Instruction for Use> 6. Preparation of Reagents> 4. Cell Lysate). What is the pH of PBS? EDTA is hardly dissolved. It’s like I prepared PBS with saturated EDTA....

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      Yes, EDTA has trouble going into solution at pH7.2. You will need to add NaOH until the pH gets to 8, and then it typically goes into solution. The safest way is with NaOH pellets but if you have an NaOH solution, you can carefully add that while monitori...

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      What are the immunogens for the antibodies in these kits?

      Thank you for your patience with this reply.

      I heard back from the lab that for the kits below, the antibodies were raised against recombinant proteins. The epitopes have not been mapped.

      1) Apolipoprotein A1 Human ELISA Kit
      2) Ap...

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      I'm interested in 3 ELISA kits. Could you please tell me what is shelf life of following ELISA kits:

      ab108804
      ab108807
      ab108813


      Here is the shelf life information you requested:
      ab108804 - 6 months if kit is unused and reagents stored at 4 deg C or -20 deg C as appropriate; 1 month after opening and limiting reagent is microtiter plate storage
      ab108807 - 6 months if...

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      Hi, I was just checking in about my question about the origin of the ApoE in the ApoE ELISA kit. Any word on when you will have the answer? Thanks for your help.

      Thank you for contacting us.

      I do want to apologize for the delay but I am still awaiting word from the supplier regarding this. I will let you know as soon as I recieve this information.



      Please let me know if you have an...

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       Hello, I have an ELISA kit for apoE and it gives data for serum and plasma at different concentrations but not for urine. Can you please tell me what concentrations the kit has been tested with for urine and if there is a recommended dilution range? I ha...

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      Thank you for contacting Abcam I have heard back from the lab and they have told me, that for urine samples you should test them at 1x (no dilution). It means that you will need to use 50 ul of urine per one well. If there is anything else I can help you ...

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