Apolipoprotein E Human ELISA Kit (ab108813)
- Product nameApolipoprotein E Human ELISA KitSee all Apolipoprotein E kits ...
- Detection methodColorimetric
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma, Cell culture extracts
- Assay typeSandwich
- Sensitivity= 0.03 µg/ml
- Range0.031 µg/ml - 2 µg/ml
- Assay time4h 00m
- Assay durationMultiple steps standard assay
- Species reactivityReacts with: Human
- Product overview
Apolipoprotein E (apoE) is a 34 kDa polymorphic protein with 299 amino acids and occurs in all lipoprotein fractions in plasma. It is synthesized primarily by the liver and is a main apoprotein of the chylomicron. ApoE is essential for the normal catabolism of triglyceride-rich lipoprotein constituents and cardiovascular diseases. ApoE is also critical in several other important biological processes, including Alzheimer's disease, cognitive function, immunoregulation, cell signaling, and infectious diseases. There are three common isoforms of the protein: apoE3 is normal; while apoE2 and apoE4 are dysfunctional. ApoE deficiency causes type III hyperlipoproteinemia and premature atherosclerosis. ApoE is a major genetic risk factor for late-onset familial Alzheimer's disease and for cognitive deficits associated with aging. ApoE4 enhances HIV-1 cell entry in vitro and the ApoE epsilon4/epsilon4 genotype accelerates HIV disease progression.
Ab108813 Human Apo E ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of human Apo E in plasma, serum, urine, and cell culture samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures Apo E in less than 4 hours. A polyclonal antibody specific for human Apo E has been pre-coated onto a 96-well microplate with removable strips. Apo E in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for Apo E, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
- Tested applicationsSandwich ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests Biotinylated Apo E Antibody 1 x 140µl Chromogen Substrate 1 x 8ml Diluent Concentrate (10x) 1 x 30ml Human Apo E Microplate 1 unit Human Apo E Standard 2 units Stop Solution 1 x 12ml Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 80µl Wash Buffer Concentrate (20x) 2 x 30ml
- Research Areas
- ApoEAPOEAPOEAApolipoprotein E3 apolipoprotein E3ApolipoproteinEApoprotein LDLCQ5LPG
Our Abpromise guarantee covers the use of ab108813 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Apolipoprotein E Human ELISA Kit (ab108813)
ab108813 has not yet been referenced specifically in any publications.