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Synthetic peptide spanning the polymorphic amino acid position 158 of ApoE.
Our Abpromise guarantee covers the use of ab1906 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 21163940|
|IP||Use at an assay dependent concentration. PubMed: 21163940|
|WB||1/1000 - 1/10000. Predicted molecular weight: 38 kDa.|
|IHC-P||1/1000 - 1/100000. Antigen retrieval is not essential but may optimise staining.
The staining intensity of formalin-fixed paraffin embedded tissues may be significantly improved by pretreatment methods such as: 70% Formic acid for 10-30 minutes at room temperature or Hydrolytic autoclaving.
|IHC-Fr||1/1000 - 1/100000.|
|Flow Cyt||Use at an assay dependent concentration. PubMed: 22896615|
ab1906 staining Apolipoprotein E in zebrafish retina sections by IHC-Fr. The tissue was fixed with paraformaldehyde and an antigen retrieval step was performed with sodium citrate pH 6. Blocking of the sample was done with 5% BSA in PBS containing 01% Tween 20 and 0.5% Triton X, for 60 minutes at 23°C, followed by staining with ab1906 at 1/100 in blocking solution for 16h at 4°C. An alexa 647 conjugated goat anti-mouset polyclonal antibody at 1/1000 was used as the secondary antibody. Nuclei are stained in blue with DAPI. Apolipoprotein E expression can be observed in Muller cells.
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