Anti-Apolipoprotein E antibody [D6E10] (ab1906)

Overview

  • Product nameAnti-Apolipoprotein E antibody [D6E10]
    See all Apolipoprotein E primary antibodies
  • Description
    Mouse monoclonal [D6E10] to Apolipoprotein E
  • SpecificityMouse reactivity: Please be aware that we have received positive as well as negative feedback for reactivity of this antibody with mouse samples. The antibody is not being batch-tested in the mouse samples.
  • Tested applicationsICC/IF, IP, WB, IHC-P, IHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human, Zebrafish
  • Immunogen

    Synthetic peptide spanning the polymorphic amino acid position 158 of ApoE.

Applications

Our Abpromise guarantee covers the use of ab1906 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 21163940
IP Use at an assay dependent concentration. PubMed: 21163940
WB 1/1000 - 1/10000. Predicted molecular weight: 38 kDa.
IHC-P 1/1000 - 1/100000. Antigen retrieval is not essential but may optimise staining.

The staining intensity of formalin-fixed paraffin embedded tissues may be significantly improved by pretreatment methods such as: 70% Formic acid for 10-30 minutes at room temperature or Hydrolytic autoclaving.

IHC-Fr 1/1000 - 1/100000.
Flow Cyt Use at an assay dependent concentration. PubMed: 22896615

Target

  • FunctionMediates the binding, internalization, and catabolism of lipoprotein particles. It can serve as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues.
  • Tissue specificityOccurs in all lipoprotein fractions in plasma. It constitutes 10-20% of very low density lipoproteins (VLDL) and 1-2% of high density lipoproteins (HDL). APOE is produced in most organs. Significant quantities are produced in liver, brain, spleen, lung, adrenal, ovary, kidney and muscle.
  • Involvement in diseaseDefects in APOE are a cause of hyperlipoproteinemia type 3 (HLPP3) [MIM:107741]; also known as familial dysbetalipoproteinemia. Individuals with HLPP3 are clinically characterized by xanthomas, yellowish lipid deposits in the palmar crease, or less specific on tendons and on elbows. The disorder rarely manifests before the third decade in men. In women, it is usually expressed only after the menopause. The vast majority of the patients are homozygous for APOE*2 alleles. More severe cases of HLPP3 have also been observed in individuals heterozygous for rare APOE variants. The influence of APOE on lipid levels is often suggested to have major implications for the risk of coronary artery disease (CAD). Individuals carrying the common APOE*4 variant are at higher risk of CAD.
    Genetic variations in APOE are associated with Alzheimer disease type 2 (AD2) [MIM:104310]. It is a late-onset neurodegenerative disorder characterized by progressive dementia, loss of cognitive abilities, and deposition of fibrillar amyloid proteins as intraneuronal neurofibrillary tangles, extracellular amyloid plaques and vascular amyloid deposits. The major constituent of these plaques is the neurotoxic amyloid-beta-APP 40-42 peptide (s), derived proteolytically from the transmembrane precursor protein APP by sequential secretase processing. The cytotoxic C-terminal fragments (CTFs) and the caspase-cleaved products such as C31 derived from APP, are also implicated in neuronal death. Note=The APOE*4 allele is genetically associated with the common late onset familial and sporadic forms of Alzheimer disease. Risk for AD increased from 20% to 90% and mean age at onset decreased from 84 to 68 years with increasing number of APOE*4 alleles in 42 families with late onset AD. Thus APOE*4 gene dose is a major risk factor for late onset AD and, in these families, homozygosity for APOE*4 was virtually sufficient to cause AD by age 80. The mechanism by which APOE*4 participates in pathogenesis is not known.
    Defects in APOE are a cause of sea-blue histiocyte disease (SBHD) [MIM:269600]; also known as sea-blue histiocytosis. This disorder is characterized by splenomegaly, mild thrombocytopenia and, in the bone marrow, numerous histiocytes containing cytoplasmic granules which stain bright blue with the usual hematologic stains. The syndrome is the consequence of an inherited metabolic defect analogous to Gaucher disease and other sphingolipidoses.
    Defects in APOE are a cause of lipoprotein glomerulopathy (LPG) [MIM:611771]. LPG is an uncommon kidney disease characterized by proteinuria, progressive kidney failure, and distinctive lipoprotein thrombi in glomerular capillaries. It mainly affects people of Japanese and Chinese origin. The disorder has rarely been described in Caucasians.
  • Sequence similaritiesBelongs to the apolipoprotein A1/A4/E family.
  • Post-translational
    modifications
    Synthesized with the sialic acid attached by O-glycosidic linkage and is subsequently desialylated in plasma. O-glycosylated with core 1 or possibly core 8 glycans. Thr-307 is a minor glycosylation site compared to Ser-308.
    Glycated in plasma VLDL of normal subjects, and of hyperglycemic diabetic patients at a higher level (2-3 fold).
    Phosphorylation sites are present in the extracelllular medium.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • AD2 antibody
    • Alzheimer disease 2 antibody
    • Apo E antibody
    • Apo-E antibody
    • ApoE antibody
    • APOE_HUMAN antibody
    • APOEA antibody
    • Apolipoprotein E antibody
    • Apolipoprotein E3 antibody
    • ApolipoproteinE antibody
    • Apoprotein antibody
    • LDLCQ5 antibody
    • LPG antibody
    • MGC1571 antibody
    see all

Anti-Apolipoprotein E antibody [D6E10] images

  • ab1906 staining Apolipoprotein E in zebrafish retina sections by IHC-Fr. The tissue was fixed with paraformaldehyde and an antigen retrieval step was performed with sodium citrate pH 6. Blocking of the sample was done with 5% BSA in PBS containing 01% Tween 20 and 0.5% Triton X, for 60 minutes at 23°C, followed by staining with ab1906 at 1/100 in blocking solution for 16h at 4°C. An alexa 647 conjugated goat anti-mouset polyclonal antibody at 1/1000 was used as the secondary antibody. Nuclei are stained in blue with DAPI. Apolipoprotein E expression can be observed in Muller cells.

    See Abreview

  • IHC-P using ab1906 showing senile plaques. (x200)
  • IHC-P using ab1906. Strong immunoreactivity to Apo E of vascular amyloid ß. Down syndrome. Orig. magn. X 100.


  • Predicted band size : 38 kDa
    Western blot using ab1906, on 1 µl of human serum. Western blot using ab1906, on 1 µl of human serum

References for Anti-Apolipoprotein E antibody [D6E10] (ab1906)

This product has been referenced in:
  • Ali-Rahmani F  et al. C282Y-HFE gene variant affects cholesterol metabolism in human neuroblastoma cells. PLoS One 9:e88724 (2014). WB ; Human . Read more (PubMed: 24533143) »
  • Lefèvre M  et al. Syndecan 4 Is Involved in Mediating HCV Entry through Interaction with Lipoviral Particle-Associated Apolipoprotein E. PLoS One 9:e95550 (2014). WB ; Human . Read more (PubMed: 24751902) »

See all 12 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Sample Zebrafish Tissue sections (Muller cell, retina)
Specification Muller cell, retina
Permeabilization Yes - 0.1% Triton X
Fixative Paraformaldehyde
Username

Dr. Ryan MacDonald

Verified customer

Submitted Nov 08 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Serum (serum)
Loading amount 100 µg
Specification serum
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Feb 15 2013

Thank you for contacting us and your interest in our products.

Unfortunately, none of the three antibodies you are interested in have been tested in in both the application you are interested in with rat samples and we do not have any other an...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Testis)
Specification Testis
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Sep 07 2012

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"