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Dear Mrs. Nordgren Thank you a lot for your help. Could you please, provide us the same details (lot nr) of our orders from year 2010? ApoE Ab were ordered twice on 18.01.10 and 24.03.10 One more time thank you Have a nice day |
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ANSWER: |
I have looked into the following orders and have found that the vials provided again came from the same stock: Order number xxxxx (PO number xxxxx) of 30th March 2010 lot number xxxxx Order number xxxxx (PO number xxxxx) of 19th of January 2010 lot number 721569 Although the lot numbers for these orders appear to be completely different they do actually come from the same stock as those of GR32116-4 and GR32116-2. Again they have been aliquotted at different times and a different numbering system was used. We have moved towards using the GR numbers in identifying individual vials as this allows us and yourselves to see quite clearly which lots are related. I hope this information has been of help. If you require any further information please do not hesitate to ask. |
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Half year ago we ordered rabbit polyclonal antibodies to Apolipoprotein E (ab20874). This product was delivered on 19. 07. 11. We performed already several experiments with this Ab. Recently (10 12 11) we reordered the same product, but as this are polyclonal Ab it would be important to know if the Lot number of this product changed since July 2011 (when the first order was placed) . Could you provide us this information? Thank you in advanced, Best Regards |
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ANSWER: |
Thank you for contacting us. I have checked through our records to find the details of your orders, as follows: 11th July 2011: Order number xxxxx (PO number xxxxx): lot number GR32116-4 13th December 2011: Order number xxxxxx (PO number xxxxxx): lot number GR32116-2 If these order details are the ones which match your deliveries, these two lots are from the same batch (animal and purification). The added -4 and -2 of the lot numbers simply indicates that the product was aliquotted by us at different times. These two products should therefore behave in the same way as each other. I hope this information has been of help. If you have any problems please do let us know. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Anti-Apolipoprotein E antibody (ab20874) + 20ug rat hippocampal lysate Rabbit polyclonal to Apolipoprotein E (ab20874) in western blot analysis of proteins isolated from 100mg of rat brain hippocampaus. Tissue was homogenized in a 5V of gentle lysis buffer (10mM HEPES, 1mM EGTA, 5mM MgCl2, 142,5mM KCl, 0,25% NP-40, with Roche complete Protease Inhibitor Cocktail Tablets) and centrifuged 20 mins/14000rpm/4oC. Supernatants reserved and protein concentrations were assigned by Bradford method. Proteins were resolved onto 10% SDS-PAGE and blotted on nitrocellulose membranes (Amersham- Hybond C). The membranes were incubated with anti-ApoE (ab20874 at 1/400) for 2h at rm temp in 0.05% TBST (150 mM NaCl, 50 mM Tris, pH 7.0, and 0.05% Tween 20) with 3% milk. Following incubation with the anti-rabbit HRP-labeled secondary antibody (1/10000 in 0.05% TBST, 1h rm temp), blots were developed using the enhanced chemiluminescent substrate (ECL, Amersham Bioscience).
Secondary
anti-rabit HRP-labeled secondary antibody
Predicted band size : 36 kDa
S Kanazir, Inst Biol Res, Yugoslavia
ab20874 staining Apolipoprotein E in rat brain tissue by Immunohistochemistry (Frozen sections). After fixation in 4% PFA in PBS (pH 7.4), brains were cryoprotected in 30% sucrose in PBS at +4ºC, frozen, and thereafter kept at -80ºC until used. For the immunostaining, brains were cryosectioned in 30 µm slices, collected in PBS (pH 7.4), and processed in a free-floating system. Slices were first incubated in the blocking solution (BS) containing 2% bovine serum albumin, 2% goat serum, and 0.1% Triton X-100 in PBS (pH 7.4) for 1 hour at room temperature, and thereafter with the primary antibody for 24–48 hours at +4ºC in BS at a 1/1000 dilution. After washing in PBS, slices were incubated with the biotin-conjugated secondary antibody (1/4000) in BS, rinsed with PBS, and incubated with the avidin-peroxidase conjugate in BS for 1 hour at room temperature. The staining was detected using 3,3-diaminobenzidinetetrahydrochloride as a chromogen.
Image from Laurén HB et al, PLoS One. 2010 May 20;5(5):e10733, Fig 5.
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