For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Full length native protein (purified) corresponding to Mouse Apolipoprotein E.
Our Abpromise guarantee covers the use of ab20874 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|RID||Use at an assay dependent concentration.|
|Immunodiffusion||Use at an assay dependent concentration.|
|Immunoelectrophoresis||Use at an assay dependent concentration.|
|WB||1/500 - 1/1000. Predicted molecular weight: 36 kDa. Dilution optimised using Chromogenic detection.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 20505763|
This image is courtesy of S Kanazir, Inst Biol Res, Yugoslavia
Rabbit polyclonal to Apolipoprotein E (ab20874) in western blot analysis of proteins isolated from 100mg of rat brain hippocampaus. Tissue was homogenized in a 5V of gentle lysis buffer (10mM HEPES, 1mM EGTA, 5mM MgCl2, 142,5mM KCl, 0,25% NP-40, with Roche complete Protease Inhibitor Cocktail Tablets) and centrifuged 20 mins/14000rpm/4oC. Supernatants reserved and protein concentrations were assigned by Bradford method. Proteins were resolved onto 10% SDS-PAGE and blotted on nitrocellulose membranes (Amersham- Hybond C). The membranes were incubated with anti-ApoE (ab20874 at 1/400) for 2h at rm temp in 0.05% TBST (150 mM NaCl, 50 mM Tris, pH 7.0, and 0.05% Tween 20) with 3% milk. Following incubation with the anti-rabbit HRP-labeled secondary antibody (1/10000 in 0.05% TBST, 1h rm temp), blots were developed using the enhanced chemiluminescent substrate (ECL, Amersham Bioscience).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"