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Full length native protein (purified)(Human) (isolated from human plasma by density gradient centrifugation followed by HPLC purification).
Our Abpromise guarantee covers the use of ab7620 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||1/4000 - 1/8000.|
|WB||1/5000 - 1/10000. Predicted molecular weight: 36 kDa.|
|ICC/IF||Use a concentration of 10 µg/ml.|
This image is courtesy of an Abreview submitted by Fabien GosseletBlocking Step: 3% Milk for 20 minutes at room temperature
ICC/IF image of ab7620 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab7620 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 donkey anti- goat (ab96931) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.